Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction

Fu De Huang; Heinrich J.G. Matthies; Sean D. Speese; Mark A. Smith; Kendal Broadie

doi:10.1038/nn1313

Rolling blackout, a newly identified PIP₂-DAG pathway lipase required for Drosophila phototransduction

Fu De Huang, Heinrich J.G. Matthies, Sean D. Speese, Mark A. Smith, Kendal Broadie

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.

Original language	English (US)
Pages (from-to)	1070-1078
Number of pages	9
Journal	Nature Neuroscience
Volume	7
Issue number	10
DOIs	https://doi.org/10.1038/nn1313
State	Published - Oct 2004
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience

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@article{0bab81cce7d14fd5a2c5eb47a58f2b2b,

title = "Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction",

abstract = "The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.",

author = "Huang, {Fu De} and Matthies, {Heinrich J.G.} and Speese, {Sean D.} and Smith, {Mark A.} and Kendal Broadie",

note = "Funding Information: We thank the Drosophila Bloomington Stock Center for supplying stocks; T. Jongens for cmp44E RFII pBluescript vector; T. Jongens, S. Chandrashekaran, C. Montell, H. Li, R. Hardie and W. Pak for flies, including cmp44E, stmA, trp343, trpl302 and norpA mutants; C. Sanders for DAG kinase; R. Shortridge for advice about PLC assays; R. Mohrmann for discussions, useful comments and help in generating figures and L. Yang for assistance in sequencing mutant alleles. This study was supported by US National Institutes of Health grant GM54544 to K.B.",

year = "2004",

month = oct,

doi = "10.1038/nn1313",

language = "English (US)",

volume = "7",

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journal = "Nature Neuroscience",

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AU - Huang, Fu De

AU - Matthies, Heinrich J.G.

AU - Speese, Sean D.

AU - Smith, Mark A.

AU - Broadie, Kendal

N1 - Funding Information: We thank the Drosophila Bloomington Stock Center for supplying stocks; T. Jongens for cmp44E RFII pBluescript vector; T. Jongens, S. Chandrashekaran, C. Montell, H. Li, R. Hardie and W. Pak for flies, including cmp44E, stmA, trp343, trpl302 and norpA mutants; C. Sanders for DAG kinase; R. Shortridge for advice about PLC assays; R. Mohrmann for discussions, useful comments and help in generating figures and L. Yang for assistance in sequencing mutant alleles. This study was supported by US National Institutes of Health grant GM54544 to K.B.

PY - 2004/10

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N2 - The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.

AB - The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.

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Rolling blackout, a newly identified PIP₂-DAG pathway lipase required for Drosophila phototransduction

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