S. cerevisiae has three pathways for DNA interstrand crosslink repair

Kenneth F. Grossmann, Alex M. Ward, Mara E. Matkovic, Alexandra E. Folias, Robb E. Moses

Research output: Contribution to journalArticlepeer-review

90 Scopus citations


Yeast mutants, snm1 (pso2-1), rev3 (pso1-1), and rad51, which display significant sensitivity to interstrand crosslinks (ICLs) have low relative sensitivity to other DNA damaging agents. SNM1, REV3, and RAD51 were disrupted in the same haploid strain, singly and in combination. The double mutants, snm1Δ rev3Δ, snm1Δ rad51Δ and rev3Δ rad51Δ were all more sensitive to ICLs than any of the single mutants, indicating that they are in separate epistasis groups for survival. A triple mutant displayed greater sensitivity to ICLs than any of the double mutants, with one ICL per genome being lethal. Therefore, Saccharomyces cerevisiae appears to have three separate ICL repair pathways, but no more. S-phase delay was not observed after ICL damage introduced by cisplatin (CDDP) or 8-methoxypsoralen (8-MOP) during the G1-phase, in any of the above mutants, or in an isogenic rad14Δ mutant deficient in nucleotide excision repair. However, the psoralen analog angelicin (monoadduct damage) induced a significant S-phase delay in the rad14Δ mutant. Thus, normal S-phase in the presence of ICLs does not seem to be due to rapid excision repair. The results also indicate that monoadduct formation by CDDP or 8-MOP at the doses used is not sufficient to delay S-phase in the rad14Δ mutant. While the sensitivity of a rev3Δ mutant indicates Polζ is needed for optimal ICL repair, isogenic cells deficient in Polη (rad30Δ cells) were not significantly more sensitive to ICL agents than wild-type cells, and have no S-phase delay.

Original languageEnglish (US)
Pages (from-to)73-83
Number of pages11
JournalMutation Research - DNA Repair
Issue number3-4
StatePublished - Dec 19 2001


  • Angelicin
  • Cisplatin
  • DNA interstrand crosslinks
  • DNA repair
  • Psoralen

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics


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