Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice

Volker Mostert; Akihiro Nakayama; Lori M. Austin; Ximena A. Levander; Christopher D. Ferris; Kristina E. Hill; Raymond F. Burk

doi:10.1080/03602530701468342

Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice

Volker Mostert, Akihiro Nakayama, Lori M. Austin, Ximena A. Levander, Christopher D. Ferris, Kristina E. Hill, Raymond F. Burk

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Heme oxygenase (HO)-1 is induced by oxidative stress and protects against oxidant injury. We examined the effect of rapid induction of hepatic HO-1 on serum iron level. Serum iron was approximately doubled within 6 h when HO-1 was induced by phenobarbital treatment of selenium-deficient mice. Blocking heme synthesis with diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate (DDC) prevented the induction of HO-1 and the rise in serum iron. DDC did not block HO-1 induction by hemin. Inhibition of HO activity by tin protoporphyrin prevented a rise in serum iron that occurred following phorone treatment. These results indicate that heme synthesis or an exogenous source of heme is needed to allow induction of HO-1. Further, they link HO-1 induction with a rise in serum iron, suggesting that the iron resulting from catabolism of heme by HO-1 is released by the liver.

Original language	English (US)
Pages (from-to)	619-626
Number of pages	8
Journal	Drug Metabolism Reviews
Volume	39
Issue number	2-3
DOIs	https://doi.org/10.1080/03602530701468342
State	Published - Apr 2007
Externally published	Yes

Keywords

Heme
Heme oxygenase-1
Heme synthesis
Iron export
Oxidative stress
Selenium deficiency

ASJC Scopus subject areas

Pharmacology, Toxicology and Pharmaceutics(all)
Pharmacology (medical)

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10.1080/03602530701468342

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title = "Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice",

abstract = "Heme oxygenase (HO)-1 is induced by oxidative stress and protects against oxidant injury. We examined the effect of rapid induction of hepatic HO-1 on serum iron level. Serum iron was approximately doubled within 6 h when HO-1 was induced by phenobarbital treatment of selenium-deficient mice. Blocking heme synthesis with diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate (DDC) prevented the induction of HO-1 and the rise in serum iron. DDC did not block HO-1 induction by hemin. Inhibition of HO activity by tin protoporphyrin prevented a rise in serum iron that occurred following phorone treatment. These results indicate that heme synthesis or an exogenous source of heme is needed to allow induction of HO-1. Further, they link HO-1 induction with a rise in serum iron, suggesting that the iron resulting from catabolism of heme by HO-1 is released by the liver.",

keywords = "Heme, Heme oxygenase-1, Heme synthesis, Iron export, Oxidative stress, Selenium deficiency",

author = "Volker Mostert and Akihiro Nakayama and Austin, {Lori M.} and Levander, {Ximena A.} and Ferris, {Christopher D.} and Hill, {Kristina E.} and Burk, {Raymond F.}",

note = "Funding Information: The authors are grateful to Dr. Maria Almira Correia for helpful discussions and for suggesting the use of DDC to block heme synthesis. The work reported here was supported by NIH grants ES02497 and ES00267. V.M. was supported by grant MO936/2-1 from the Deutsche Forschungsgemeinschaft.",

year = "2007",

month = apr,

doi = "10.1080/03602530701468342",

language = "English (US)",

volume = "39",

pages = "619--626",

journal = "Drug Metabolism Reviews",

issn = "0360-2532",

publisher = "Informa Healthcare",

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TY - JOUR

T1 - Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice

AU - Mostert, Volker

AU - Nakayama, Akihiro

AU - Austin, Lori M.

AU - Levander, Ximena A.

AU - Ferris, Christopher D.

AU - Hill, Kristina E.

AU - Burk, Raymond F.

N1 - Funding Information: The authors are grateful to Dr. Maria Almira Correia for helpful discussions and for suggesting the use of DDC to block heme synthesis. The work reported here was supported by NIH grants ES02497 and ES00267. V.M. was supported by grant MO936/2-1 from the Deutsche Forschungsgemeinschaft.

PY - 2007/4

Y1 - 2007/4

N2 - Heme oxygenase (HO)-1 is induced by oxidative stress and protects against oxidant injury. We examined the effect of rapid induction of hepatic HO-1 on serum iron level. Serum iron was approximately doubled within 6 h when HO-1 was induced by phenobarbital treatment of selenium-deficient mice. Blocking heme synthesis with diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate (DDC) prevented the induction of HO-1 and the rise in serum iron. DDC did not block HO-1 induction by hemin. Inhibition of HO activity by tin protoporphyrin prevented a rise in serum iron that occurred following phorone treatment. These results indicate that heme synthesis or an exogenous source of heme is needed to allow induction of HO-1. Further, they link HO-1 induction with a rise in serum iron, suggesting that the iron resulting from catabolism of heme by HO-1 is released by the liver.

AB - Heme oxygenase (HO)-1 is induced by oxidative stress and protects against oxidant injury. We examined the effect of rapid induction of hepatic HO-1 on serum iron level. Serum iron was approximately doubled within 6 h when HO-1 was induced by phenobarbital treatment of selenium-deficient mice. Blocking heme synthesis with diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate (DDC) prevented the induction of HO-1 and the rise in serum iron. DDC did not block HO-1 induction by hemin. Inhibition of HO activity by tin protoporphyrin prevented a rise in serum iron that occurred following phorone treatment. These results indicate that heme synthesis or an exogenous source of heme is needed to allow induction of HO-1. Further, they link HO-1 induction with a rise in serum iron, suggesting that the iron resulting from catabolism of heme by HO-1 is released by the liver.

KW - Heme

KW - Heme oxygenase-1

KW - Heme synthesis

KW - Iron export

KW - Oxidative stress

KW - Selenium deficiency

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ER -

Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice

Abstract

Keywords

ASJC Scopus subject areas

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