TY - JOUR
T1 - Simian retrovirus serogroup 5
T2 - Partial gag-prt sequence and viral RNA distribution in an infected rhesus macaque
AU - Li, B.
AU - Axthelm, M. K.
AU - Machida, C. A.
N1 - Funding Information:
The authors acknowledge Heidi Palmer, Chong-Fang Ju, Tarsem Moudgil, and Kirsten Pilcher for their technical assistance in the provision of cells used to develop the Northern analyses. We thank Dr. Daniel K. Rohrer (formerly associated with the Department of Neurosurgery, OHSU; currently in private practice with Microneurosurgical Associates, Portland, OR) for conducting the brain region dissections. Technical assistance from Susan Nguyen is also gratefully acknowledged for work conducted during latter stages of this project. We thank Yibing Jia for his assistance in the synthesis of oligonucleotide primers and for automated sequencing support, provided as a service from the ORPRC Molecular and Cell Biology Core Facility. We thank Dr. Cynthia Bethea and the U54 Primate Tissue Bank (NIH-NICHD U54 18185; Specialized Cooperative Centers in Reproductive Research Grant), for provision of seminal vesicle and prostate tissues from uninfected rhesus macaques. We also thank Dr. Anne Lewis (Chief, Pathology, ORPRC/OHSU) for her assistance in confirming the uninfected status of these animals, and the ORPRC Virology Services Laboratory, including Heldi Palmer, for their original monitoring of the virus load and serum antibodies present in the D5/RHE/OR-infected rhesus macaque during the near-six year infection period. We also thank other current members of the Machida Laboratory, including Philbert Kirigiti, Ying Bai, Xiaorong Li, Matt Palmer, and Luz Pell for encouragement and support. LB was a 1997 Leukemia Research Foundation Postdoctoral Fellow. MKA is supported by NCRR 00163. This research has been supported by grants awarded to CAM from NIH DK53462, the Medical Research Foundation of Oregon, and the Collins Medical Trust, and by core grant projects awarded to CAM and MKA from NCRR 00163. CAM is supported by NCRR 00163, NIH HL42358, and NIH DK53462, and was a former recipient of an American Heart Association Established Investigatorship.
PY - 2000
Y1 - 2000
N2 - The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.
AB - The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.
KW - Dot blot
KW - Generalized lymphoid hyperplasia
KW - Northern blot analyses
KW - Partial sequence of D5/RHE/OR gag-prt product
KW - Partial sequence of rhesus macaque cyclophilin product
KW - Reverse transcriptase-polymerase chain reaction (RT-PCR)
KW - Simian retrovirus (SRV) serogroup 5
KW - Tissue distribution analyses of D5/RHE/OR RNA
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U2 - 10.1023/A:1008104000920
DO - 10.1023/A:1008104000920
M3 - Article
C2 - 11129642
AN - SCOPUS:0033672099
SN - 0920-8569
VL - 21
SP - 241
EP - 248
JO - Virus Genes
JF - Virus Genes
IS - 3
ER -