Simian retrovirus serogroup 5: Partial gag-prt sequence and viral RNA distribution in an infected rhesus macaque

B. Li, M. K. Axthelm, C. A. Machida

    Research output: Contribution to journalArticlepeer-review

    17 Scopus citations


    The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.

    Original languageEnglish (US)
    Pages (from-to)241-248
    Number of pages8
    JournalVirus Genes
    Issue number3
    StatePublished - 2000


    • Dot blot
    • Generalized lymphoid hyperplasia
    • Northern blot analyses
    • Partial sequence of D5/RHE/OR gag-prt product
    • Partial sequence of rhesus macaque cyclophilin product
    • Reverse transcriptase-polymerase chain reaction (RT-PCR)
    • Simian retrovirus (SRV) serogroup 5
    • Tissue distribution analyses of D5/RHE/OR RNA

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Virology


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