Single-Cell Analysis in Cerebrovascular Research: Primed for Breakthroughs and Clinical Impact

Asher J. Albertson, Ethan A. Winkler, Andrew C. Yang, Marion S. Buckwalter, Andra L. Dingman, Huihui Fan, Paco S. Herson, Louise D. McCullough, Miguel A. Perez-Pinzon, Lauren H. Sansing, Dandan Sun, Nabil J. Alkayed

Research output: Contribution to journalReview articlepeer-review

Abstract

Data generated using single-cell RNA-sequencing has the potential to transform understanding of the cerebral circulation and advance clinical care. However, the high volume of data, sometimes generated and presented without proper pathophysiological context, can be difficult to interpret and integrate into current understanding of the cerebral circulation and its disorders. Furthermore, heterogeneity in the representation of brain regions and vascular segments makes it difficult to compare results across studies. There are currently no standards for tissue collection and processing that allow easy comparisons across studies and analytical platforms. There are no standards either for single-cell data analysis and presentation. This topical review introduces single-cell RNA-sequencing to physicians and scientists in the cerebrovascular field, with the goals of highlighting opportunities and challenges of applying this technology in the cerebrovascular field and discussing key concepts and knowledge gaps that can be addressed by single-cell RNA-sequencing.

Original languageEnglish (US)
Pages (from-to)1082-1091
Number of pages10
JournalStroke
Volume56
Issue number4
DOIs
StatePublished - Apr 1 2025

Keywords

  • brain
  • cerebrovascular circulation
  • sequence analysis, RNA
  • stroke

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

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