Single cell cloning of Leishmania parasites in purine-defined medium: isolation of drug-resistant variants.

D. M. Iovannisci; B. Ullman

doi:10.1007/978-1-4684-4553-4_46

Single cell cloning of Leishmania parasites in purine-defined medium: isolation of drug-resistant variants.

D. M. Iovannisci, B. Ullman

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A simple technique for the isolation of Leishmania donovani and Leishmania tropica promastigote clones derived from a single cell involves the use of semi-solid agar. Both species of Leishmania form discrete colonies at high efficiency in completely defined medium lacking serum. Visible colonies appear between eight and fourteen days. Viability of colonies transferred from semi-solid agar to liquid suspension culture is 100%. Using these techniques, we have isolated clonal populations of cells resistant to tubercidin and formycin b.

Original language	English (US)
Pages (from-to)	239-243
Number of pages	5
Journal	Advances in experimental medicine and biology
Volume	165 Pt A
DOIs	https://doi.org/10.1007/978-1-4684-4553-4_46
State	Published - 1984
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1007/978-1-4684-4553-4_46

Cite this

@article{5b36b22666cc40449ddd0e9adb59eb67,

title = "Single cell cloning of Leishmania parasites in purine-defined medium: isolation of drug-resistant variants.",

abstract = "A simple technique for the isolation of Leishmania donovani and Leishmania tropica promastigote clones derived from a single cell involves the use of semi-solid agar. Both species of Leishmania form discrete colonies at high efficiency in completely defined medium lacking serum. Visible colonies appear between eight and fourteen days. Viability of colonies transferred from semi-solid agar to liquid suspension culture is 100%. Using these techniques, we have isolated clonal populations of cells resistant to tubercidin and formycin b.",

author = "Iovannisci, {D. M.} and B. Ullman",

year = "1984",

doi = "10.1007/978-1-4684-4553-4_46",

language = "English (US)",

volume = "165 Pt A",

pages = "239--243",

journal = "Advances in experimental medicine and biology",

issn = "0065-2598",

publisher = "Springer New York",

}

TY - JOUR

T1 - Single cell cloning of Leishmania parasites in purine-defined medium

T2 - isolation of drug-resistant variants.

AU - Iovannisci, D. M.

AU - Ullman, B.

PY - 1984

Y1 - 1984

N2 - A simple technique for the isolation of Leishmania donovani and Leishmania tropica promastigote clones derived from a single cell involves the use of semi-solid agar. Both species of Leishmania form discrete colonies at high efficiency in completely defined medium lacking serum. Visible colonies appear between eight and fourteen days. Viability of colonies transferred from semi-solid agar to liquid suspension culture is 100%. Using these techniques, we have isolated clonal populations of cells resistant to tubercidin and formycin b.

AB - A simple technique for the isolation of Leishmania donovani and Leishmania tropica promastigote clones derived from a single cell involves the use of semi-solid agar. Both species of Leishmania form discrete colonies at high efficiency in completely defined medium lacking serum. Visible colonies appear between eight and fourteen days. Viability of colonies transferred from semi-solid agar to liquid suspension culture is 100%. Using these techniques, we have isolated clonal populations of cells resistant to tubercidin and formycin b.

UR - http://www.scopus.com/inward/record.url?scp=0021298256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021298256&partnerID=8YFLogxK

U2 - 10.1007/978-1-4684-4553-4_46

DO - 10.1007/978-1-4684-4553-4_46

M3 - Article

C2 - 6720386

AN - SCOPUS:0021298256

SN - 0065-2598

VL - 165 Pt A

SP - 239

EP - 243

JO - Advances in experimental medicine and biology

JF - Advances in experimental medicine and biology

ER -

Single cell cloning of Leishmania parasites in purine-defined medium: isolation of drug-resistant variants.

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this