TY - JOUR
T1 - Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma
AU - Blise, Katie E.
AU - Sivagnanam, Shamilene
AU - Banik, Grace L.
AU - Coussens, Lisa M.
AU - Goecks, Jeremy
N1 - Funding Information:
The authors thank Dr. Courtney Betts, Justin Tibbitts, Teresa Beechwood, and Meghan Lavoie for regulatory and technical assistance. The authors thank the OHSU Knight Biostatistics Shared Resource for guidance with statistical calculations. The authors also acknowledge and thank the patients who donated tissue samples for this study. K.E.B. acknowledges funding from the National Cancer Institute (NCI) of the National Institutes of Health under award number T32CA254888. L.M.C. acknowledges funding from the NCI awards U01CA224012, R01CA223150, R01CA226909, and R21HD099367 and funding from the Brenden-Colson Center for Pancreatic Care at OHSU. J.G. acknowledges funding under award number U24CA231877. L.M.C. and J.G. acknowledge funding from the OHSU Knight Cancer Institute, NCI award number U2CCA233280, and the Prospect Creek Foundation to the OHSU SMMART (Serial Measurement of Molecular and Architectural Responses to Therapy) Program.
Funding Information:
The authors thank Dr. Courtney Betts, Justin Tibbitts, Teresa Beechwood, and Meghan Lavoie for regulatory and technical assistance. The authors thank the OHSU Knight Biostatistics Shared Resource for guidance with statistical calculations. The authors also acknowledge and thank the patients who donated tissue samples for this study. K.E.B. acknowledges funding from the National Cancer Institute (NCI) of the National Institutes of Health under award number T32CA254888. L.M.C. acknowledges funding from the NCI awards U01CA224012, R01CA223150, R01CA226909, and R21HD099367 and funding from the Brenden-Colson Center for Pancreatic Care at OHSU. J.G. acknowledges funding under award number U24CA231877. L.M.C. and J.G. acknowledge funding from the OHSU Knight Cancer Institute, NCI award number U2CCA233280, and the Prospect Creek Foundation to the OHSU SMMART (Serial Measurement of Molecular and Architectural Responses to Therapy) Program.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - There is increasing evidence that the spatial organization of cells within the tumor-immune microenvironment (TiME) of solid tumors influences survival and response to therapy in numerous cancer types. Here, we report results and demonstrate the applicability of quantitative single-cell spatial proteomics analyses in the TiME of primary and recurrent human papillomavirus (HPV)-negative head and neck squamous cell carcinoma (HNSCC) tumors. Single-cell compositions of a nine patient, primary and recurrent (n = 18), HNSCC cohort is presented, followed by deeper investigation into the spatial architecture of the TiME and its relationship with clinical variables and progression free survival (PFS). Multiple spatial algorithms were used to quantify the spatial landscapes of immune cells within TiMEs and demonstrate that neoplastic tumor-immune cell spatial compartmentalization, rather than mixing, is associated with longer PFS. Mesenchymal (αSMA+) cellular neighborhoods describe distinct immune landscapes associated with neoplastic tumor-immune compartmentalization and improved patient outcomes. Results from this investigation are concordant with studies in other tumor types, suggesting that trends in TiME cellular heterogeneity and spatial organization may be shared across cancers and may provide prognostic value in multiple cancer types.
AB - There is increasing evidence that the spatial organization of cells within the tumor-immune microenvironment (TiME) of solid tumors influences survival and response to therapy in numerous cancer types. Here, we report results and demonstrate the applicability of quantitative single-cell spatial proteomics analyses in the TiME of primary and recurrent human papillomavirus (HPV)-negative head and neck squamous cell carcinoma (HNSCC) tumors. Single-cell compositions of a nine patient, primary and recurrent (n = 18), HNSCC cohort is presented, followed by deeper investigation into the spatial architecture of the TiME and its relationship with clinical variables and progression free survival (PFS). Multiple spatial algorithms were used to quantify the spatial landscapes of immune cells within TiMEs and demonstrate that neoplastic tumor-immune cell spatial compartmentalization, rather than mixing, is associated with longer PFS. Mesenchymal (αSMA+) cellular neighborhoods describe distinct immune landscapes associated with neoplastic tumor-immune compartmentalization and improved patient outcomes. Results from this investigation are concordant with studies in other tumor types, suggesting that trends in TiME cellular heterogeneity and spatial organization may be shared across cancers and may provide prognostic value in multiple cancer types.
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U2 - 10.1038/s41698-022-00253-z
DO - 10.1038/s41698-022-00253-z
M3 - Article
AN - SCOPUS:85125525832
SN - 2397-768X
VL - 6
JO - npj Precision Oncology
JF - npj Precision Oncology
IS - 1
M1 - 10
ER -