Single microtubules from squid axoplasm support bidirectional movement of organelles

Bruce J. Schnapp; Ronald D. Vale; Michael P. Sheetz; Thomas S. Reese

doi:10.1016/0092-8674(85)90160-6

Single microtubules from squid axoplasm support bidirectional movement of organelles

Bruce J. Schnapp
, Ronald D. Vale
, Michael P. Sheetz
, Thomas S. Reese

Research output: Contribution to journal › Article › peer-review

257 Scopus citations

Abstract

Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.

Original language	English (US)
Pages (from-to)	455-462
Number of pages	8
Journal	Cell
Volume	40
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(85)90160-6
State	Published - Feb 1985
Externally published	Yes

Funding

We would like to thank John Murphy for help with the preparation of the figures. R. D. V. is a trainee in the Medical Scientist Training Program supported by the National Institutes of Health (GM 07365). This work was supported in part by grants from Hoechst-Rousselle Pharmaceuticals and NIH (GM 33351) to M. P S., who is an established investigator of the American Heart Association.

Funders	Funder number
Hoechst-Rousselle Pharmaceuticals
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	GM 33351
National Institute of General Medical Sciences	T32GM007365
American Heart Association/American Stroke Association

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(85)90160-6

Cite this

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title = "Single microtubules from squid axoplasm support bidirectional movement of organelles",

abstract = "Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.",

author = "Schnapp, \{Bruce J.\} and Vale, \{Ronald D.\} and Sheetz, \{Michael P.\} and Reese, \{Thomas S.\}",

note = "Funding Information: We would like to thank John Murphy for help with the preparation of the figures. R. D. V. is a trainee in the Medical Scientist Training Program supported by the National Institutes of Health (GM 07365). This work was supported in part by grants from Hoechst-Rousselle Pharmaceuticals and NIH (GM 33351) to M. P S., who is an established investigator of the American Heart Association.",

year = "1985",

month = feb,

doi = "10.1016/0092-8674(85)90160-6",

language = "English (US)",

volume = "40",

pages = "455--462",

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T1 - Single microtubules from squid axoplasm support bidirectional movement of organelles

AU - Schnapp, Bruce J.

AU - Vale, Ronald D.

AU - Sheetz, Michael P.

AU - Reese, Thomas S.

N1 - Funding Information: We would like to thank John Murphy for help with the preparation of the figures. R. D. V. is a trainee in the Medical Scientist Training Program supported by the National Institutes of Health (GM 07365). This work was supported in part by grants from Hoechst-Rousselle Pharmaceuticals and NIH (GM 33351) to M. P S., who is an established investigator of the American Heart Association.

PY - 1985/2

Y1 - 1985/2

N2 - Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.

AB - Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.

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Single microtubules from squid axoplasm support bidirectional movement of organelles

Abstract

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ASJC Scopus subject areas

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