Abstract
Multipotent adult progenitor cells (MAPCs) are adult adherent stromal stem cells currently being assessedin clinical trials foracutegraft versus host disease with demonstrated im munomodulatory capabilities and the potential to ameliorate detrimental autoimmune and inflammation-related processes. Anti-CD3/anti-CD28(3/28) activation of T cells within the peripheral blood mononuclear cell (PBMC) compartmentwas performed in the presence or absence of MAPCs. Liquid chromatography-coupled tandem mass spectrometry was used to characterize the differential secretion of proteins, and transcriptional profiling was used to monitorm RNA expression changes in both cell populations. Overall, 239 secreted and/or ectodomain-shed proteins were detected in the secretomes of PBMCs and MAPCs. In addition, 3/28 activation of PBMCs induced differential expression of 2,925 genes, and 22% of these transcripts were differentially expressed on exposure to MAPCs in Transwell. MAPCs exposed to 3/28-activated PBMCs showed differential expression of 1,247MAPC genes. Crosstalk was demonstrated by reciprocal transcriptional regulation. Secretome proteins and transcriptional signatures were used to predict molecular activities by which MAPCs could dampen local and systemic inflammatory responses. These data support the hypothesis that MAPCs block PBMC proliferation via cell cycle arrest coupled to metabolic stress in the form of tryptophan depletion, resulting in GCN2 kinase activation, downstream signaling, and inhibition of cyclin D1 translation. These data also provide a plausible explanation for the immune privilege reported with administration of donor MAPCs. Although most components of the major histocompatibility complex class II antigen presentation pathway were markedly transcriptionally upregulated, cell surface expression of human leukocyte antigen-DR is minimal on MAPCs exposed to 3/28-activated PBMCs.
Original language | English (US) |
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Pages (from-to) | 1436-1449 |
Number of pages | 14 |
Journal | Stem Cells Translational Medicine |
Volume | 4 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2015 |
Keywords
- Anti-CD3/anti-CD28-activated PBMC
- Cell cycle arrest
- Immune tolerance
- MRNA gene array
- Multipotent adult progenitor cell
ASJC Scopus subject areas
- General Medicine