Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult β-globin gene

B. M. Emerson; G. Felsenfeld

doi:10.1073/pnas.81.1.95

Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult β-globin gene

B. M. Emerson, G. Felsenfeld

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Abstract

Chromatin in the 5' flanking region of the transcriptionally active chicken adult β (β(A) globin genes is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the β(A)-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

Original language	English (US)
Pages (from-to)	95-99
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	81
Issue number	1 I
DOIs	https://doi.org/10.1073/pnas.81.1.95
State	Published - 1984
Externally published	Yes

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title = "Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult β-globin gene",

abstract = "Chromatin in the 5' flanking region of the transcriptionally active chicken adult β (β(A) globin genes is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the β(A)-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.",

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year = "1984",

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T1 - Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult β-globin gene

AU - Emerson, B. M.

AU - Felsenfeld, G.

PY - 1984

Y1 - 1984

N2 - Chromatin in the 5' flanking region of the transcriptionally active chicken adult β (β(A) globin genes is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the β(A)-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

AB - Chromatin in the 5' flanking region of the transcriptionally active chicken adult β (β(A) globin genes is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the β(A)-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

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JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 1 I

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Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult β-globin gene

Abstract

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