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Spectral fractionation detection of gold nanorod contrast agents using optical coherence tomography

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate the proof of concept of a novel Fourier-domain optical coherence tomography contrast mechanism using gold nanorod contrast agents and a spectral fractionation processing technique. The methodology detects the spectral shift of the backscattered light from the nanorods by comparing the ratio between the short and long wavelength halves of the optical coherence tomography signal intensity. Spectral fractionation further divides the halves into sub-bands to improve spectral contrast and suppress speckle noise. Herein, we show that this technique can detect gold nanorods in intralipid tissue phantoms. Furthermore, cellular labeling by gold nanorods was demonstrated using retinal pigment epithelial cells in vitro.

Original languageEnglish (US)
Pages (from-to)4212-4225
Number of pages14
JournalOptics express
Volume23
Issue number4
DOIs
StatePublished - Feb 23 2015

Funding

FundersFunder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of HealthT32EY23211, R01EY024544, R01EY023285, R01EY023397
National Eye Institute and Casey Eye InstituteT32EY007135

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics

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