Structure and organization of the chicken H2B histone gene family

David K. Grandy; Jerry B. Dodgson

doi:10.1093/nar/15.3.1063

Structure and organization of the chicken H2B histone gene family

David K. Grandy
, Jerry B. Dodgson

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The results of Southern blotting experiments confirm that the chicken H2B histone gene family contains eight highly homologous members. One or two more sequences which are considerably divergent from the others appear to exist in the chicken genome. Seven of the eight H2B genes have been cloned and sequenced. All seven genes fall in two histone gene clusters, but no common arrangement exists for the clusters themselves. Three different H2B protein variants are encoded by these seven genes. The nucleotide sequence homology among the genes within their coding sequences appears to exceed that required for the corresponding protein sequences, suggesting that histone H2B mRNA sequence and structure are both selected during evolution. An analysis of the 5′ flanking sequence data reveals that these genes possess CCAAT and TATA boxes, elements commonly associated with genes transcribed by RNA polymerase II. In addition, these genes all share an H2B-specific element of the form: ATTTGCATA. The 3′ sequences of these genes contain the hyphenated symmetrical dyad homology and downstream purine-rich sequence shared by histone genes in general.

Original language	English (US)
Pages (from-to)	1063-1080
Number of pages	18
Journal	Nucleic acids research
Volume	15
Issue number	3
DOIs	https://doi.org/10.1093/nar/15.3.1063
State	Published - Feb 11 1987
Externally published	Yes

Funding

ACKNOWLEDGEMENTS We thank Seung-Yeol Son and David Browne of our lab for access to unpublished results. We also thank Barry Sugarman and J. D. Engel (Northwestern U.) for sharing ideas and results prior to publication. This research was supported by grant GM 29687 and by a Research Career Development Award to J.B.D., both from the National Institutes of Health. Initial stages of the work were supported by Grant 1-0759 from the March of Dime3 Birth Defects Foundation. Journal Article No. 11919 from the Michigan Agricultural Experiment Station.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	1-0759

ASJC Scopus subject areas

Genetics

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10.1093/nar/15.3.1063

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title = "Structure and organization of the chicken H2B histone gene family",

abstract = "The results of Southern blotting experiments confirm that the chicken H2B histone gene family contains eight highly homologous members. One or two more sequences which are considerably divergent from the others appear to exist in the chicken genome. Seven of the eight H2B genes have been cloned and sequenced. All seven genes fall in two histone gene clusters, but no common arrangement exists for the clusters themselves. Three different H2B protein variants are encoded by these seven genes. The nucleotide sequence homology among the genes within their coding sequences appears to exceed that required for the corresponding protein sequences, suggesting that histone H2B mRNA sequence and structure are both selected during evolution. An analysis of the 5′ flanking sequence data reveals that these genes possess CCAAT and TATA boxes, elements commonly associated with genes transcribed by RNA polymerase II. In addition, these genes all share an H2B-specific element of the form: ATTTGCATA. The 3′ sequences of these genes contain the hyphenated symmetrical dyad homology and downstream purine-rich sequence shared by histone genes in general.",

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note = "Funding Information: ACKNOWLEDGEMENTS We thank Seung-Yeol Son and David Browne of our lab for access to unpublished results. We also thank Barry Sugarman and J. D. Engel (Northwestern U.) for sharing ideas and results prior to publication. This research was supported by grant GM 29687 and by a Research Career Development Award to J.B.D., both from the National Institutes of Health. Initial stages of the work were supported by Grant 1-0759 from the March of Dime3 Birth Defects Foundation. Journal Article No. 11919 from the Michigan Agricultural Experiment Station.",

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N2 - The results of Southern blotting experiments confirm that the chicken H2B histone gene family contains eight highly homologous members. One or two more sequences which are considerably divergent from the others appear to exist in the chicken genome. Seven of the eight H2B genes have been cloned and sequenced. All seven genes fall in two histone gene clusters, but no common arrangement exists for the clusters themselves. Three different H2B protein variants are encoded by these seven genes. The nucleotide sequence homology among the genes within their coding sequences appears to exceed that required for the corresponding protein sequences, suggesting that histone H2B mRNA sequence and structure are both selected during evolution. An analysis of the 5′ flanking sequence data reveals that these genes possess CCAAT and TATA boxes, elements commonly associated with genes transcribed by RNA polymerase II. In addition, these genes all share an H2B-specific element of the form: ATTTGCATA. The 3′ sequences of these genes contain the hyphenated symmetrical dyad homology and downstream purine-rich sequence shared by histone genes in general.

AB - The results of Southern blotting experiments confirm that the chicken H2B histone gene family contains eight highly homologous members. One or two more sequences which are considerably divergent from the others appear to exist in the chicken genome. Seven of the eight H2B genes have been cloned and sequenced. All seven genes fall in two histone gene clusters, but no common arrangement exists for the clusters themselves. Three different H2B protein variants are encoded by these seven genes. The nucleotide sequence homology among the genes within their coding sequences appears to exceed that required for the corresponding protein sequences, suggesting that histone H2B mRNA sequence and structure are both selected during evolution. An analysis of the 5′ flanking sequence data reveals that these genes possess CCAAT and TATA boxes, elements commonly associated with genes transcribed by RNA polymerase II. In addition, these genes all share an H2B-specific element of the form: ATTTGCATA. The 3′ sequences of these genes contain the hyphenated symmetrical dyad homology and downstream purine-rich sequence shared by histone genes in general.

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Structure and organization of the chicken H2B histone gene family

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