Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement

Haixiao Gao, Jayati Sengupta, Mikel Valle, Andrei Korostelev, Narayanan Eswar, Scott M. Stagg, Patrick Van Roey, Rajendra K. Agrawal, Stephen C. Harvey, Andrej Sali, Michael S. Chapman, Joachim Frank

Research output: Contribution to journalArticlepeer-review

253 Scopus citations


Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.

Original languageEnglish (US)
Pages (from-to)789-801
Number of pages13
Issue number6
StatePublished - Jun 13 2003
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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