Styrene-maleic acid copolymer effects on the function of the GPCR rhodopsin in lipid nanoparticles

Istvan Szundi; Stephanie G. Pitch; Eefei Chen; David L. Farrens; David S. Kliger

doi:10.1016/j.bpj.2021.09.012

Styrene-maleic acid copolymer effects on the function of the GPCR rhodopsin in lipid nanoparticles

Istvan Szundi
, Stephanie G. Pitch
, Eefei Chen
, David L. Farrens
, David S. Kliger

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Styrene-maleic acid (SMA) copolymers solubilize biological membranes to form lipid nanoparticles (SMALPs) that contain membrane proteins surrounded by native lipids, thus enabling the use of a variety of biophysical techniques for structural and functional studies. The question of whether SMALPs provide a truly natural environment or SMA solubilization affects the functional properties of membrane proteins, however, remains open. We address this question by comparing the photoactivation kinetics of rhodopsin, a G-protein-coupled receptor in the disk membranes of rod cells, in native membrane and SMALPs prepared at different molar ratios between SMA(3:1) and rhodopsin. Time-resolved absorption spectroscopy combined with complex kinetic analysis reveals kinetic and mechanistic differences between the native membrane and SMA-stabilized environment. The results suggest a range of molar ratios for nanoparticles suitable for kinetic studies.

Original language	English (US)
Pages (from-to)	4337-4348
Number of pages	12
Journal	Biophysical Journal
Volume	120
Issue number	20
DOIs	https://doi.org/10.1016/j.bpj.2021.09.012
State	Published - Oct 19 2021
Externally published	Yes

Funding

This work was supported by National Institutes of Health Grant R01EY029343 (D.S.K. and D.L.F.).

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health
National Eye Institute and Casey Eye Institute	R01EY029343

ASJC Scopus subject areas

Biophysics

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10.1016/j.bpj.2021.09.012

Cite this

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title = "Styrene-maleic acid copolymer effects on the function of the GPCR rhodopsin in lipid nanoparticles",

abstract = "Styrene-maleic acid (SMA) copolymers solubilize biological membranes to form lipid nanoparticles (SMALPs) that contain membrane proteins surrounded by native lipids, thus enabling the use of a variety of biophysical techniques for structural and functional studies. The question of whether SMALPs provide a truly natural environment or SMA solubilization affects the functional properties of membrane proteins, however, remains open. We address this question by comparing the photoactivation kinetics of rhodopsin, a G-protein-coupled receptor in the disk membranes of rod cells, in native membrane and SMALPs prepared at different molar ratios between SMA(3:1) and rhodopsin. Time-resolved absorption spectroscopy combined with complex kinetic analysis reveals kinetic and mechanistic differences between the native membrane and SMA-stabilized environment. The results suggest a range of molar ratios for nanoparticles suitable for kinetic studies.",

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AU - Szundi, Istvan

AU - Pitch, Stephanie G.

AU - Chen, Eefei

AU - Farrens, David L.

AU - Kliger, David S.

PY - 2021/10/19

Y1 - 2021/10/19

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AB - Styrene-maleic acid (SMA) copolymers solubilize biological membranes to form lipid nanoparticles (SMALPs) that contain membrane proteins surrounded by native lipids, thus enabling the use of a variety of biophysical techniques for structural and functional studies. The question of whether SMALPs provide a truly natural environment or SMA solubilization affects the functional properties of membrane proteins, however, remains open. We address this question by comparing the photoactivation kinetics of rhodopsin, a G-protein-coupled receptor in the disk membranes of rod cells, in native membrane and SMALPs prepared at different molar ratios between SMA(3:1) and rhodopsin. Time-resolved absorption spectroscopy combined with complex kinetic analysis reveals kinetic and mechanistic differences between the native membrane and SMA-stabilized environment. The results suggest a range of molar ratios for nanoparticles suitable for kinetic studies.

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Styrene-maleic acid copolymer effects on the function of the GPCR rhodopsin in lipid nanoparticles

Abstract

Funding

ASJC Scopus subject areas

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