Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

Jonathan C. Savage; Monika A. Davare; Ujwal Shinde

doi:10.1039/c9cc09375c

Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

Jonathan C. Savage, Monika A. Davare, Ujwal Shinde

Pediatric Hematology and Oncology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex-cation interactions in RNA.

Original language	English (US)
Pages (from-to)	2634-2637
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	17
DOIs	https://doi.org/10.1039/c9cc09375c
State	Published - Feb 28 2020

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex-cation interactions in RNA.",

author = "Savage, {Jonathan C.} and Davare, {Monika A.} and Ujwal Shinde",

note = "Funding Information: The Authors thank Dr Dave Farrens for the use of his spectroscopic equipment. This work was supported by the NIH (J. C. S) and the Oregon MRF (U. S.). Publisher Copyright: This journal is {\textcopyright} The Royal Society of Chemistry.",

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AU - Davare, Monika A.

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AB - Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex-cation interactions in RNA.

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Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

Abstract

ASJC Scopus subject areas

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