Abstract
Chondrocytes were dissociated enzymatically from canine nucleus pulposus. After attaining monolayer growth, the cells were subpassaged and incubated with sodium salicylate, indomethacin, or sodium meclofenamate (10-2 M to 3 x 10˜6 M). When incubated with nonsteroidal antiinflammatory drug concentrations mimicking therapeutic plasma levels, proteoglycan synthesis was significantly suppressed. After 24 hours of incubation, there was a 15% decrease in radioactive sulfate (35S04) incorporation for salicylate (10-3 M), a 29% de¬crease for indomethacin (3 x 10-6 M), and a 75% decrease for sodium meclofenamate (10“5 M). This inhibition was both dose and timedependent. Despite the effect of these nonsteroidal anti-inflammatory drugs on quantitative proteoglycan synthesis, there was no demonstrable effect of these drugs on the size of proteoglycan monomers or the degree to which proteoglycan aggregate formation occurred.
Original language | English (US) |
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Pages (from-to) | 221-224 |
Number of pages | 4 |
Journal | Spine |
Volume | 17 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1992 |
Keywords
- Nonsteroidal anti-inflammatory drugs
- Nucleus pulposus chondrocytes
- Proteoglycan synthesis
ASJC Scopus subject areas
- Orthopedics and Sports Medicine
- Clinical Neurology