TY - JOUR
T1 - Susceptibility of primary culture neurons from rats of different ages to encephalomyocarditis (EMC) virus infection
AU - Su, Weiping
AU - Ikegami, Hisashi
AU - Nakayama, Yumi
AU - Suzuki, Kazuhiko
AU - Katayama, Kei Ichi
AU - Nakayama, Hiroyuki
AU - Doi, Kunio
PY - 2003/10
Y1 - 2003/10
N2 - The changes in susceptibility of neurons to the D variant of EMC virus (EMC-D) (106 PFU/well) were investigated in developing hippocampal primary cultures from postnatal days of 1, 7, and 56 Fischer 344 rats (P1, P7, and P56) for up to 12 h after infection (12 HAI). The virus titer of primary culture neurons increased at 1 HAI, decreased at 2 HAI, increased at 3 HAI, peaked at 8 HAI, and decreased at 12 HAI in all age groups. The titers at 1 and 8 HAI were lowest in P56 cultures. The virus titer of neurons was always higher than that of culture media, especially at 1 HAI, in P1 cultures, whereas the former was lower than the latter from 2 to 3 HAI in P7 cultures and from 2 to 4 HAI in P56 cultures, respectively. Signals of viral RNA detected by in situ hybridization were first observed in the peripheral cytoplasm of neurons at 1 HAI in P1 and P7 cultures and at 4 HAI in P56 cultures, respectively. The signals spread to a large or whole area of cytoplasm and also to processes thereafter. The number of viral RNA-positive neurons and the amount of signals decreased with age. The present results indicated that the susceptibility of primary culture neurons to EMC-D decreased with age but viral replication still occurred in P56 cultures.
AB - The changes in susceptibility of neurons to the D variant of EMC virus (EMC-D) (106 PFU/well) were investigated in developing hippocampal primary cultures from postnatal days of 1, 7, and 56 Fischer 344 rats (P1, P7, and P56) for up to 12 h after infection (12 HAI). The virus titer of primary culture neurons increased at 1 HAI, decreased at 2 HAI, increased at 3 HAI, peaked at 8 HAI, and decreased at 12 HAI in all age groups. The titers at 1 and 8 HAI were lowest in P56 cultures. The virus titer of neurons was always higher than that of culture media, especially at 1 HAI, in P1 cultures, whereas the former was lower than the latter from 2 to 3 HAI in P7 cultures and from 2 to 4 HAI in P56 cultures, respectively. Signals of viral RNA detected by in situ hybridization were first observed in the peripheral cytoplasm of neurons at 1 HAI in P1 and P7 cultures and at 4 HAI in P56 cultures, respectively. The signals spread to a large or whole area of cytoplasm and also to processes thereafter. The number of viral RNA-positive neurons and the amount of signals decreased with age. The present results indicated that the susceptibility of primary culture neurons to EMC-D decreased with age but viral replication still occurred in P56 cultures.
KW - Age
KW - Encephalomyocarditis virus
KW - Primary culture neuron
KW - Rat
KW - Susceptibility
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U2 - 10.1016/S0014-4800(03)00068-6
DO - 10.1016/S0014-4800(03)00068-6
M3 - Article
C2 - 14516779
AN - SCOPUS:0141739487
SN - 0014-4800
VL - 75
SP - 160
EP - 164
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 2
ER -