Synthesis and radiobiological applications of [35S]l-homocysteine thiolactone

Zdenka Grunbaum; Keith Kroll; Janet L. Greene; Janet S. Rasey; Kenneth A. Krohn

doi:10.1016/0883-2897(90)90166-X

Synthesis and radiobiological applications of [³⁵S]l-homocysteine thiolactone

Zdenka Grunbaum, Keith Kroll, Janet L. Greene, Janet S. Rasey, Kenneth A. Krohn

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

[³⁵S]l-Homocysteine thiolactone ([³⁵S]l-HCTL) was synthesized and its biodistribution evaluated as a potential brain radioprotective agent and as a tissue hypoxia marker. Drug uptake in mouse brain exceeded that in s.c. tumor 3 h post injection only. Multiple indicator dilution experiments in the rabbit heart indicate that membrane permeability of [³⁵S]l-HCTL does not limit its usefulness as a hypoxia marker. In addition, a positive correlation was observed between regional coronary blood flow and myocardial content of [³⁵S]adenosylhomocysteine formed from [³⁵S]homocysteine and adenosine.

Original language	English (US)
Pages (from-to)	473-478
Number of pages	6
Journal	International Journal of Radiation Applications and Instrumentation.
Volume	17
Issue number	5
DOIs	https://doi.org/10.1016/0883-2897(90)90166-X
State	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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10.1016/0883-2897(90)90166-X

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@article{6e6948bbda334fa28fe64cba2775dc0e,

title = "Synthesis and radiobiological applications of [35S]l-homocysteine thiolactone",

abstract = "[35S]l-Homocysteine thiolactone ([35S]l-HCTL) was synthesized and its biodistribution evaluated as a potential brain radioprotective agent and as a tissue hypoxia marker. Drug uptake in mouse brain exceeded that in s.c. tumor 3 h post injection only. Multiple indicator dilution experiments in the rabbit heart indicate that membrane permeability of [35S]l-HCTL does not limit its usefulness as a hypoxia marker. In addition, a positive correlation was observed between regional coronary blood flow and myocardial content of [35S]adenosylhomocysteine formed from [35S]homocysteine and adenosine.",

author = "Zdenka Grunbaum and Keith Kroll and Greene, {Janet L.} and Rasey, {Janet S.} and Krohn, {Kenneth A.}",

note = "Funding Information: Acknowledgements-Waer eg ratefutl o Dr A. M. Spencefo r stimulatingo ur interestin normalb rainr adioprotectiona nd his readinessto act as our consultant.T echnicale xcellence of Norma Nelsona nd Lay Chin is gratefullya cknowledged. This work was supportedi n part by ROlCA34570 and ROlCA36485 NIH, DHHS, and the SimulationR esource Facility for Circulatory Mass Transport and Exchange, RR1243N IH. DRR.",

year = "1990",

doi = "10.1016/0883-2897(90)90166-X",

language = "English (US)",

volume = "17",

pages = "473--478",

journal = "International Journal of Radiation Applications and Instrumentation.",

issn = "0883-2897",

publisher = "Elsevier Inc.",

number = "5",

}

TY - JOUR

T1 - Synthesis and radiobiological applications of [35S]l-homocysteine thiolactone

AU - Grunbaum, Zdenka

AU - Kroll, Keith

AU - Greene, Janet L.

AU - Rasey, Janet S.

AU - Krohn, Kenneth A.

N1 - Funding Information: Acknowledgements-Waer eg ratefutl o Dr A. M. Spencefo r stimulatingo ur interestin normalb rainr adioprotectiona nd his readinessto act as our consultant.T echnicale xcellence of Norma Nelsona nd Lay Chin is gratefullya cknowledged. This work was supportedi n part by ROlCA34570 and ROlCA36485 NIH, DHHS, and the SimulationR esource Facility for Circulatory Mass Transport and Exchange, RR1243N IH. DRR.

PY - 1990

Y1 - 1990

N2 - [35S]l-Homocysteine thiolactone ([35S]l-HCTL) was synthesized and its biodistribution evaluated as a potential brain radioprotective agent and as a tissue hypoxia marker. Drug uptake in mouse brain exceeded that in s.c. tumor 3 h post injection only. Multiple indicator dilution experiments in the rabbit heart indicate that membrane permeability of [35S]l-HCTL does not limit its usefulness as a hypoxia marker. In addition, a positive correlation was observed between regional coronary blood flow and myocardial content of [35S]adenosylhomocysteine formed from [35S]homocysteine and adenosine.

AB - [35S]l-Homocysteine thiolactone ([35S]l-HCTL) was synthesized and its biodistribution evaluated as a potential brain radioprotective agent and as a tissue hypoxia marker. Drug uptake in mouse brain exceeded that in s.c. tumor 3 h post injection only. Multiple indicator dilution experiments in the rabbit heart indicate that membrane permeability of [35S]l-HCTL does not limit its usefulness as a hypoxia marker. In addition, a positive correlation was observed between regional coronary blood flow and myocardial content of [35S]adenosylhomocysteine formed from [35S]homocysteine and adenosine.

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U2 - 10.1016/0883-2897(90)90166-X

DO - 10.1016/0883-2897(90)90166-X

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VL - 17

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EP - 478

JO - International Journal of Radiation Applications and Instrumentation.

JF - International Journal of Radiation Applications and Instrumentation.

IS - 5

ER -

Synthesis and radiobiological applications of [³⁵S]l-homocysteine thiolactone

Abstract

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