TY - JOUR
T1 - TaCRK3 encodes a novel Theileria annulata protein kinase with motifs characteristic of the family of eukaryotic cyclin dependent kinases
T2 - A comparative analysis of its expression with TaCRK2 during the parasite life cycle
AU - Kinnaird, Jane
AU - Logan, Mary
AU - Tait, Andrew
AU - Langsley, Gordon
N1 - Funding Information:
We would like to thank Dr Frank Katzer for use of his T. annulata piroplasm cDNA library, Dr Christian Doerig for helpful and critical reading of the manuscript and Dr Brian Shiels for his unfailing support and advice. We would also like to thank the Wellcome Trust and the BBSRC for funding and Duncan Brown and colleagues at the CTVM, Edinburgh, for supplying parasite-infected blood.
PY - 2001/11/28
Y1 - 2001/11/28
N2 - The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.
AB - The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.
KW - Apicomplexa
KW - Cyclin-dependent kinases
KW - Differentiation
KW - Nuclear localization
KW - Theileria
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U2 - 10.1016/S0378-1119(01)00753-3
DO - 10.1016/S0378-1119(01)00753-3
M3 - Article
C2 - 11733137
AN - SCOPUS:0035965775
SN - 0378-1119
VL - 279
SP - 127
EP - 135
JO - Gene
JF - Gene
IS - 2
ER -