@inbook{a4b7d970daec40f79e0be782aeecf691,
title = "Tagmentation-based library preparation for low DNA input whole genome bisulfite sequencing",
abstract = "Aberrations of the DNA methylome contribute to onset and progression of diseases. Whole genome bisulfite sequencing (WGBS) is the only analytical method covering the complete methylome. Alternative methods requiring less DNA than WGBS analyze only a minor portion of the methylome and do not cover important regulatory features like enhancers and noncoding RNAs. In tagmentation-based WGBS (TWGBS), several DNA and time-consuming steps of the conventional WGBS library preparation are circumvented by the use of a hyperactive transposase, which simultaneously fragments DNA and appends sequencing adapters. TWGBS requires only nanogram amounts of DNA and, thus, is well suited to study precious biological specimens such as sorted cells or micro-dissected tissue samples.",
keywords = "5-Methyl cytosine, Methylome, Tagmentation, Transposase, Whole genome bisulfite sequencing",
author = "Dieter Weichenhan and Qi Wang and Andrew Adey and Stephan Wolf and Jay Shendure and Roland Eils and Christoph Plass",
note = "Funding Information: We gratefully acknowledge excellent technical support by Marion B€ahr, Monika Helf and helpful discussions with Daniel Lipka. We also acknowledge the excellent support from the sequencing core facility at the DKFZ, particularly from Andr{\'e} G{\"o}tze. Work in the Plass laboratory was supported by the Helmholtz Foundation and the German Federal Ministry of Education and Science in the program for medical genome research (FKZ: 01KU1001A). Q.W. obtained support by the Humboldt Research Fellowship for Postdoctoral Researchers. A.A. is funded by an NSF Graduate Research Fellowship. Author Contributions: D.W., Q.W., A. A. and J.S. conceived the study. S.W., R.E. and C.P. contributed materials. D.W. did the experiments and analyzed data. D.W. and C.P. wrote the manuscript. Competing financial interests: The authors declare no competing financial interests. A provisional patent application has been deposited for aspects of these methods (A.A., J.S.). Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC.",
year = "2018",
doi = "10.1007/978-1-4939-7481-8_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "105--122",
booktitle = "Methods in Molecular Biology",
}