@article{dd521e1fa01543c8aab8455b570395e0,
title = "Terumo spectra optia leukapheresis of cynomolgus macaques for hematopoietic stem cell and T cell collection",
abstract = "Macaques are physiologically relevant animal models of human immunology and infectious disease that have provided key insights and advanced clinical treatment in transplantation, vaccinology, and HIV/AIDS. However, the small size of macaques is a stumbling block for studies requiring large numbers of cells, such as hematopoietic stem cells (HSCs) for transplantation, antigen-specific lymphocytes for in-depth immunological analysis, and latently-infected CD4+ T-cells for HIV cure studies. Here, we provide a detailed protocol for collection of large numbers of HSCs and T-cells from cynomolgus macaques as small as 3 kg using the Terumo Spectra Optia apheresis system, yielding an average of 5.0 × 109 total nucleated cells from mobilized animals and 1.2 × 109 total nucleated cells from nonmobilized animals per procedure. This report provides sufficient detail to adapt this apheresis technique at other institutions, which will facilitate more efficient and detailed analysis of HSCs and their progeny blood cells.",
keywords = "Leukapheresis, cynomolgus macaques, hematopoietic stem cells, leukocytes",
author = "Wu, {Helen L.} and Greene, {Justin M.} and Tonya Swanson and Christine Shriver-Munsch and Kimberly Armantrout and Weber, {Whitney C.} and Bateman, {Katherine B.} and Maier, {Nicholas M.} and Mina Northrup and Legasse, {Alfred W.} and Cassandra Moats and Axthelm, {Michael K.} and Jeremy Smedley and Maziarz, {Richard T.} and Martin, {Lauren Drew} and Theodore Hobbs and Burwitz, {Benjamin J.} and Sacha, {Jonah B.}",
note = "Funding Information: We gratefully acknowledge Amgen, Inc. for providing G‐CSF for this study. This work was supported by National Institutes of Health grants R21 AI112433, R01 AI129703, and R01 AI140888 awarded to J.B.S., K01 OD026561 awarded to J.M.G., and P51 OD011092 awarded to the Oregon National Primate Research Center. Funding Information: We gratefully acknowledge Amgen, Inc. for providing G-CSF for this study. This work was supported by National Institutes of Health grants R21 AI112433, R01 AI129703, and R01 AI140888 awarded to J.B.S., K01 OD026561 awarded to J.M.G., and P51 OD011092 awarded to the Oregon National Primate Research Center. Publisher Copyright: {\textcopyright} 2020 Wiley Periodicals LLC",
year = "2021",
month = feb,
doi = "10.1002/jca.21842",
language = "English (US)",
volume = "36",
pages = "67--77",
journal = "Journal of Clinical Apheresis",
issn = "0733-2459",
publisher = "Wiley-Liss Inc.",
number = "1",
}