The β-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae

Xiaorong Li; Robb E. Moses

doi:10.1016/S1568-7864(02)00192-1

The β-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae

Xiaorong Li, Robb E. Moses

Molecular and Medical Genetics

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The SNM1 gene of Saccharomyces cerevisiae is specific for repair of DNA interstrand crosslinks (ICLs). We report that the SNM1 gene functions in steps needed for the reformation of chromosomal DNA after double-strand breaks (DSBs) made in the process of ICL repair. However, SNM1 function is not needed for repair of HO endonuclease-generated DSBs. Therefore, the function of the SNM1 gene appears to act in the processing of the intermediates of the DSB repair, since the rate and extent of DSB appearance after ICL formation is normal in mutants lacking SNM1 function. The action of the SNM1 gene does not appear to depend on homologous recombination, but it does depend on an intact β-lactamase domain conserved with Artemis, a protein required for processing of V(D)J recombination intermediates.

Original language	English (US)
Pages (from-to)	121-129
Number of pages	9
Journal	DNA Repair
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/S1568-7864(02)00192-1
State	Published - Jan 2 2003

Keywords

DNA repair
HO endonuclease
Interstrand crosslinks
S. cerevisiae
SNM1
β-Lacatamase

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1016/S1568-7864(02)00192-1

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title = "The β-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae",

abstract = "The SNM1 gene of Saccharomyces cerevisiae is specific for repair of DNA interstrand crosslinks (ICLs). We report that the SNM1 gene functions in steps needed for the reformation of chromosomal DNA after double-strand breaks (DSBs) made in the process of ICL repair. However, SNM1 function is not needed for repair of HO endonuclease-generated DSBs. Therefore, the function of the SNM1 gene appears to act in the processing of the intermediates of the DSB repair, since the rate and extent of DSB appearance after ICL formation is normal in mutants lacking SNM1 function. The action of the SNM1 gene does not appear to depend on homologous recombination, but it does depend on an intact β-lactamase domain conserved with Artemis, a protein required for processing of V(D)J recombination intermediates.",

keywords = "DNA repair, HO endonuclease, Interstrand crosslinks, S. cerevisiae, SNM1, β-Lacatamase",

author = "Xiaorong Li and Moses, {Robb E.}",

note = "Funding Information: This work was supported by a grant from the National Institutes of Health, National Institute of General Medical Sciences (GM58186). We thank M. Cai, B. Ferguson, and J. Haber for providing yeast strains and plasmids and James Hejna and Lori Thrun for helpful discussion and critical reading.",

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doi = "10.1016/S1568-7864(02)00192-1",

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T1 - The β-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae

AU - Li, Xiaorong

AU - Moses, Robb E.

N1 - Funding Information: This work was supported by a grant from the National Institutes of Health, National Institute of General Medical Sciences (GM58186). We thank M. Cai, B. Ferguson, and J. Haber for providing yeast strains and plasmids and James Hejna and Lori Thrun for helpful discussion and critical reading.

PY - 2003/1/2

Y1 - 2003/1/2

N2 - The SNM1 gene of Saccharomyces cerevisiae is specific for repair of DNA interstrand crosslinks (ICLs). We report that the SNM1 gene functions in steps needed for the reformation of chromosomal DNA after double-strand breaks (DSBs) made in the process of ICL repair. However, SNM1 function is not needed for repair of HO endonuclease-generated DSBs. Therefore, the function of the SNM1 gene appears to act in the processing of the intermediates of the DSB repair, since the rate and extent of DSB appearance after ICL formation is normal in mutants lacking SNM1 function. The action of the SNM1 gene does not appear to depend on homologous recombination, but it does depend on an intact β-lactamase domain conserved with Artemis, a protein required for processing of V(D)J recombination intermediates.

AB - The SNM1 gene of Saccharomyces cerevisiae is specific for repair of DNA interstrand crosslinks (ICLs). We report that the SNM1 gene functions in steps needed for the reformation of chromosomal DNA after double-strand breaks (DSBs) made in the process of ICL repair. However, SNM1 function is not needed for repair of HO endonuclease-generated DSBs. Therefore, the function of the SNM1 gene appears to act in the processing of the intermediates of the DSB repair, since the rate and extent of DSB appearance after ICL formation is normal in mutants lacking SNM1 function. The action of the SNM1 gene does not appear to depend on homologous recombination, but it does depend on an intact β-lactamase domain conserved with Artemis, a protein required for processing of V(D)J recombination intermediates.

KW - DNA repair

KW - HO endonuclease

KW - Interstrand crosslinks

KW - S. cerevisiae

KW - SNM1

KW - β-Lacatamase

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The β-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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