The autoreactive T cell population in experimental allergic encephalomyelitis: T cell receptor β-chain rearrangements

Mary Pat Happ; Aniko S. Kiraly; Halina Offner; Arthur Vandenbark; Ellen Heber-Katz

doi:10.1016/0165-5728(88)90002-1

The autoreactive T cell population in experimental allergic encephalomyelitis: T cell receptor β-chain rearrangements

Mary Pat Happ, Aniko S. Kiraly, Halina Offner, Arthur Vandenbark, Ellen Heber-Katz

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

T cell receptor β-chain gene rearrangements were examined in myelin basic protein (MBP)-reactive T cell lines and hybridomas from Lewis (Lew) rats. Nearly 75% of the cloned hybrids were specific for the major encephalitogenic determinant residues 68-88 of guinea pig (GP) MBP; three fine specificities could be distinguished. Southern blot analysis of receptor β-chain genes revealed polyclonality and shared rearrangements not seen in non-68-88-specific clones. Generation of short-term, encephalitogenic Lew T cell lines revealed rearrangements shared with the 68-88-specific hybrids, indicating that the hybrids were representative of the whole antigen-specific population and suggesting restricted V_β gene usage within this polyclonal population.

Original language	English (US)
Pages (from-to)	191-204
Number of pages	14
Journal	Journal of Neuroimmunology
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/0165-5728(88)90002-1
State	Published - Sep 1988
Externally published	Yes

Keywords

(Guinea pig)
Experimental allergic encephalomyelitis
Major histocompatibility complex
Myelin basic protein
T cell receptor β-chain

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/0165-5728(88)90002-1

Cite this

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title = "The autoreactive T cell population in experimental allergic encephalomyelitis: T cell receptor β-chain rearrangements",

abstract = "T cell receptor β-chain gene rearrangements were examined in myelin basic protein (MBP)-reactive T cell lines and hybridomas from Lewis (Lew) rats. Nearly 75% of the cloned hybrids were specific for the major encephalitogenic determinant residues 68-88 of guinea pig (GP) MBP; three fine specificities could be distinguished. Southern blot analysis of receptor β-chain genes revealed polyclonality and shared rearrangements not seen in non-68-88-specific clones. Generation of short-term, encephalitogenic Lew T cell lines revealed rearrangements shared with the 68-88-specific hybrids, indicating that the hybrids were representative of the whole antigen-specific population and suggesting restricted Vβ gene usage within this polyclonal population.",

keywords = "(Guinea pig), Experimental allergic encephalomyelitis, Major histocompatibility complex, Myelin basic protein, T cell receptor β-chain",

author = "Happ, {Mary Pat} and Kiraly, {Aniko S.} and Halina Offner and Arthur Vandenbark and Ellen Heber-Katz",

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AU - Happ, Mary Pat

AU - Kiraly, Aniko S.

AU - Offner, Halina

AU - Vandenbark, Arthur

AU - Heber-Katz, Ellen

PY - 1988/9

Y1 - 1988/9

N2 - T cell receptor β-chain gene rearrangements were examined in myelin basic protein (MBP)-reactive T cell lines and hybridomas from Lewis (Lew) rats. Nearly 75% of the cloned hybrids were specific for the major encephalitogenic determinant residues 68-88 of guinea pig (GP) MBP; three fine specificities could be distinguished. Southern blot analysis of receptor β-chain genes revealed polyclonality and shared rearrangements not seen in non-68-88-specific clones. Generation of short-term, encephalitogenic Lew T cell lines revealed rearrangements shared with the 68-88-specific hybrids, indicating that the hybrids were representative of the whole antigen-specific population and suggesting restricted Vβ gene usage within this polyclonal population.

AB - T cell receptor β-chain gene rearrangements were examined in myelin basic protein (MBP)-reactive T cell lines and hybridomas from Lewis (Lew) rats. Nearly 75% of the cloned hybrids were specific for the major encephalitogenic determinant residues 68-88 of guinea pig (GP) MBP; three fine specificities could be distinguished. Southern blot analysis of receptor β-chain genes revealed polyclonality and shared rearrangements not seen in non-68-88-specific clones. Generation of short-term, encephalitogenic Lew T cell lines revealed rearrangements shared with the 68-88-specific hybrids, indicating that the hybrids were representative of the whole antigen-specific population and suggesting restricted Vβ gene usage within this polyclonal population.

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KW - T cell receptor β-chain

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The autoreactive T cell population in experimental allergic encephalomyelitis: T cell receptor β-chain rearrangements

Abstract

Keywords

ASJC Scopus subject areas

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