Abstract
Purpose: Having observed that sperm from the chronically obstructed caput epididymis fertilize poorly in vitro, we investigated the effect of partial zona dissection (PZD) on fertilization in a murine model of unilateral proximal epididymal obstruction. Cleavage rates were compared for zona-intact oocytes and PZD oocytes incubated with sperm from the following epididymal segments: the obstructed caput, the contralateral nonobstructed caput, the contralateral cauda, and a sperm-free preparation to control for parthenogenesis. Results: Unilateral epididymal obstruction resulted in significantly higher sperm counts in the obstructed caput compared to the nonobstructed caput, although there was no difference in motility. Cleavage rates for ova incubated with sperm from the obstructed caput and the nonobstructed caput were uniformly poor and did not differ significantly from those for the sperm-free controls. Cauda sperm fertilized significantly better than all other sperm groups (P<0.05). Partial zona dissection did not improve cleavage rates in any group. Conclusion: We conclude that sperm from the chronically obstructed caput epididymis, like sperm from the normal caput, are unable to fertilize ova, and PZD does not enhance this poor fertilizing capacity. Furthermore, the finding that PZD does not improve the fertilizing capacity of the presumably mature cauda sperm in our mouse model suggests that any beneficial effect of PZD may be strain-specific.
Original language | English (US) |
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Pages (from-to) | 43-48 |
Number of pages | 6 |
Journal | Journal of Assisted Reproduction and Genetics |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1994 |
Externally published | Yes |
Keywords
- epididymis
- in vitro fertilization
- infertility
- micromanipulation
- spermatozoa
ASJC Scopus subject areas
- Reproductive Medicine
- Genetics
- Obstetrics and Gynecology
- Developmental Biology
- Genetics(clinical)