The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function

Yosuke Iizumi; Hiroshi Sagara; Yasuaki Kabe; Motoki Azuma; Kanako Kume; Michinaga Ogawa; Takeshi Nagai; Peter G. Gillespie; Chihiro Sasakawa; Hiroshi Handa

doi:10.1016/j.chom.2007.09.012

The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function

Yosuke Iizumi
, Hiroshi Sagara
, Yasuaki Kabe
, Motoki Azuma
, Kanako Kume
, Michinaga Ogawa
, Takeshi Nagai
, Peter G. Gillespie
, Chihiro Sasakawa
, Hiroshi Handa

Otolaryngology

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

Enteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the cytoplasm of host cells. However, the physiological functions of EspB within the host cell cytoplasm remain unclear. We show that EspB binds to myosins, which are a superfamily of proteins that interact with actin filaments and mediate essential cellular processes, including microvillus formation and phagocytosis. EspB inhibits the interaction of myosins with actin, and an EspB mutant that lacks the myosin-binding region maintained its TTSS function but could not induce microvillus effacing or suppress phagocytosis. Moreover, the myosin-binding region of EspB is essential for Citrobacter rodentium, an EPEC-related murine pathogen, to efficiently infect mice. These results suggest that EspB inhibits myosin functions and thereby facilitates efficient infection by EPEC.

Original language	English (US)
Pages (from-to)	383-392
Number of pages	10
Journal	Cell Host and Microbe
Volume	2
Issue number	6
DOIs	https://doi.org/10.1016/j.chom.2007.09.012
State	Published - Dec 13 2007

Funding

We would like to thank Toshihiko Suzuki for providing the bone marrow-derived macrophages and Yasunori Tsuboi for technical support. This work was supported by Special Coordination Funds for Promoting Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology of Japan and by a grant from the New Energy and Industrial Technology Development Organization (NEDO), Japan.

Funders
Japan Ministry of Education, Culture, Sports, Science and Technology
New Energy and Industrial Technology Development Organization

Keywords

CELLBIO
MICROBIO

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

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10.1016/j.chom.2007.09.012

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title = "The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function",

abstract = "Enteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the cytoplasm of host cells. However, the physiological functions of EspB within the host cell cytoplasm remain unclear. We show that EspB binds to myosins, which are a superfamily of proteins that interact with actin filaments and mediate essential cellular processes, including microvillus formation and phagocytosis. EspB inhibits the interaction of myosins with actin, and an EspB mutant that lacks the myosin-binding region maintained its TTSS function but could not induce microvillus effacing or suppress phagocytosis. Moreover, the myosin-binding region of EspB is essential for Citrobacter rodentium, an EPEC-related murine pathogen, to efficiently infect mice. These results suggest that EspB inhibits myosin functions and thereby facilitates efficient infection by EPEC.",

keywords = "CELLBIO, MICROBIO",

author = "Yosuke Iizumi and Hiroshi Sagara and Yasuaki Kabe and Motoki Azuma and Kanako Kume and Michinaga Ogawa and Takeshi Nagai and Gillespie, \{Peter G.\} and Chihiro Sasakawa and Hiroshi Handa",

note = "Funding Information: We would like to thank Toshihiko Suzuki for providing the bone marrow-derived macrophages and Yasunori Tsuboi for technical support. This work was supported by Special Coordination Funds for Promoting Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology of Japan and by a grant from the New Energy and Industrial Technology Development Organization (NEDO), Japan.",

year = "2007",

month = dec,

day = "13",

doi = "10.1016/j.chom.2007.09.012",

language = "English (US)",

volume = "2",

pages = "383--392",

journal = "Cell Host and Microbe",

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T1 - The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function

AU - Iizumi, Yosuke

AU - Sagara, Hiroshi

AU - Kabe, Yasuaki

AU - Azuma, Motoki

AU - Kume, Kanako

AU - Ogawa, Michinaga

AU - Nagai, Takeshi

AU - Gillespie, Peter G.

AU - Sasakawa, Chihiro

AU - Handa, Hiroshi

N1 - Funding Information: We would like to thank Toshihiko Suzuki for providing the bone marrow-derived macrophages and Yasunori Tsuboi for technical support. This work was supported by Special Coordination Funds for Promoting Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology of Japan and by a grant from the New Energy and Industrial Technology Development Organization (NEDO), Japan.

PY - 2007/12/13

Y1 - 2007/12/13

N2 - Enteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the cytoplasm of host cells. However, the physiological functions of EspB within the host cell cytoplasm remain unclear. We show that EspB binds to myosins, which are a superfamily of proteins that interact with actin filaments and mediate essential cellular processes, including microvillus formation and phagocytosis. EspB inhibits the interaction of myosins with actin, and an EspB mutant that lacks the myosin-binding region maintained its TTSS function but could not induce microvillus effacing or suppress phagocytosis. Moreover, the myosin-binding region of EspB is essential for Citrobacter rodentium, an EPEC-related murine pathogen, to efficiently infect mice. These results suggest that EspB inhibits myosin functions and thereby facilitates efficient infection by EPEC.

AB - Enteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the cytoplasm of host cells. However, the physiological functions of EspB within the host cell cytoplasm remain unclear. We show that EspB binds to myosins, which are a superfamily of proteins that interact with actin filaments and mediate essential cellular processes, including microvillus formation and phagocytosis. EspB inhibits the interaction of myosins with actin, and an EspB mutant that lacks the myosin-binding region maintained its TTSS function but could not induce microvillus effacing or suppress phagocytosis. Moreover, the myosin-binding region of EspB is essential for Citrobacter rodentium, an EPEC-related murine pathogen, to efficiently infect mice. These results suggest that EspB inhibits myosin functions and thereby facilitates efficient infection by EPEC.

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The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function

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