TY - JOUR
T1 - The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research
AU - So, Seongjun
AU - Lee, Yeonmi
AU - Choi, Jiwan
AU - Kang, Seoon
AU - Lee, Ji Yoon
AU - Hwang, Julie
AU - Shin, Joosung
AU - Dutton, James R.
AU - Seo, Eul Ju
AU - Lee, Beom Hee
AU - Kim, Chong Jai
AU - Mitalipov, Shoukhrat
AU - Oh, Soo Jin
AU - Kang, Eunju
N1 - Publisher Copyright:
© 2020 So et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2020/5
Y1 - 2020/5
N2 - Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research.
AB - Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research.
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U2 - 10.1371/journal.pone.0233057
DO - 10.1371/journal.pone.0233057
M3 - Article
C2 - 32396545
AN - SCOPUS:85084532434
SN - 1932-6203
VL - 15
JO - PloS one
JF - PloS one
IS - 5
M1 - e0233057
ER -