The sequence of human serum albumin cDNA and its expression in E. coli

Richard M. Lawn; John Adelman; Susan C. Bock; Arthur E. Franke; Catherine M. Houck; Richard Najarian; Peter H. Seeburg; Karen L. Wion

doi:10.1093/nar/9.22.6103

The sequence of human serum albumin cDNA and its expression in E. coli

Richard M. Lawn
, John Adelman
, Susan C. Bock
, Arthur E. Franke
, Catherine M. Houck
, Richard Najarian
, Peter H. Seeburg
, Karen L. Wion

Research output: Contribution to journal › Article › peer-review

164 Scopus citations

Abstract

A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control of the E. coli trp promoter-operator. The DNA sequence and predicted protein sequence of HSA were determined from the cDNA plasmid and are compared to existing data obtained from direct protein sequencing. The DNA sequence predicts a mature protein of 585 amino acids preceded by a 24 amino acid "prepro' peptide.

Original language	English (US)
Pages (from-to)	6103-6114
Number of pages	12
Journal	Nucleic acids research
Volume	9
Issue number	22
DOIs	https://doi.org/10.1093/nar/9.22.6103
State	Published - Nov 25 1981
Externally published	Yes

Funding

We wish to thank Daniel Palermo for valuable participation in early phases of this project, Stephen Rowe for procuring tissue samples, Roberto Crea, Mark Vasser andcoworkers for oligonucleotide synthesis, and Jeanne Arch and Alane Gray for assistance in manuscript and illustration preparation, respectively. This research was supported by Genentech, Inc.

Funders
Genentech

ASJC Scopus subject areas

Genetics

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10.1093/nar/9.22.6103

Cite this

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title = "The sequence of human serum albumin cDNA and its expression in E. coli",

abstract = "A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control of the E. coli trp promoter-operator. The DNA sequence and predicted protein sequence of HSA were determined from the cDNA plasmid and are compared to existing data obtained from direct protein sequencing. The DNA sequence predicts a mature protein of 585 amino acids preceded by a 24 amino acid {"}prepro' peptide.",

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doi = "10.1093/nar/9.22.6103",

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AU - Lawn, Richard M.

AU - Adelman, John

AU - Bock, Susan C.

AU - Franke, Arthur E.

AU - Houck, Catherine M.

AU - Najarian, Richard

AU - Seeburg, Peter H.

AU - Wion, Karen L.

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Y1 - 1981/11/25

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The sequence of human serum albumin cDNA and its expression in E. coli

Abstract

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