TY - JOUR
T1 - The stromal cell marker SPARC predicts for survival in patients with diffuse large B-cell lymphoma treated with rituximab
AU - Meyer, Paul N.
AU - Fu, Kai
AU - Greiner, Timothy
AU - Smith, Lynette
AU - Delabie, Jan
AU - Gascoyne, Randy
AU - Ott, German
AU - Rosenwald, Andreas
AU - Braziel, Rita
AU - Campo, Elias
AU - Vose, Julie
AU - Lenz, Georg
AU - Staudt, Louis
AU - Chan, Wing
AU - Weisenburger, Dennis D.
PY - 2011/1
Y1 - 2011/1
N2 - The cellular composition of the tumor microenvironment may affect survival in diffuse large B-cell lymphoma (DLBCL). We performed immunostains for 2 stromal cell markers, CD68 and SPARC (secreted protein, acidic and rich in cysteine), in 262 patients with DLBCL treated with rituximab and cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) or CHOP-like therapies. Patients with any SPARC+ cells in the microenvironment had a significantly longer overall survival, and patients with high SPARC positivity in the microenvironment also had a significantly longer event-free survival. Survival differences were mainly due to the prognostic effect of SPARC+ cells in activated B-cell (ABC)-type DLBCL, with no effect found in the germinal center B-cell-type DLBCL. Of clinical features examined, only the number of extranodal sites was significantly associated with SPARC expression. Multivariate analysis revealed that SPARC expression predicted patient survival independent of the International Prognostic Index or tumor cell of origin. SPARC expression in the microenvironment of DLBCL can be used for prognostic purposes, determining a subgroup of patients with ABC DLBCL who have significantly longer survival. More aggressive chemotherapy protocols should be considered for patients with ABC DLBCL without SPARC+ stromal cells. CD68 expression by cells in the microenvironment did not predict survival.
AB - The cellular composition of the tumor microenvironment may affect survival in diffuse large B-cell lymphoma (DLBCL). We performed immunostains for 2 stromal cell markers, CD68 and SPARC (secreted protein, acidic and rich in cysteine), in 262 patients with DLBCL treated with rituximab and cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) or CHOP-like therapies. Patients with any SPARC+ cells in the microenvironment had a significantly longer overall survival, and patients with high SPARC positivity in the microenvironment also had a significantly longer event-free survival. Survival differences were mainly due to the prognostic effect of SPARC+ cells in activated B-cell (ABC)-type DLBCL, with no effect found in the germinal center B-cell-type DLBCL. Of clinical features examined, only the number of extranodal sites was significantly associated with SPARC expression. Multivariate analysis revealed that SPARC expression predicted patient survival independent of the International Prognostic Index or tumor cell of origin. SPARC expression in the microenvironment of DLBCL can be used for prognostic purposes, determining a subgroup of patients with ABC DLBCL who have significantly longer survival. More aggressive chemotherapy protocols should be considered for patients with ABC DLBCL without SPARC+ stromal cells. CD68 expression by cells in the microenvironment did not predict survival.
KW - CD68
KW - Diffuse large B-cell lymphoma
KW - Microenvironment
KW - Prognosis
KW - SPARC
KW - Stromal cells
KW - Tumor markers, biological
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UR - http://www.scopus.com/inward/citedby.url?scp=79251502962&partnerID=8YFLogxK
U2 - 10.1309/AJCPJX4BJV9NLQHY
DO - 10.1309/AJCPJX4BJV9NLQHY
M3 - Article
C2 - 21173124
AN - SCOPUS:79251502962
SN - 0002-9173
VL - 135
SP - 54
EP - 61
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 1
ER -