The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold

Debanu Das; Piotr Kozbial; Gye Won Han; Dennis Carlton; Lukasz Jaroszewski; Polat Abdubek; Tamara Astakhova; Herbert L. Axelrod; Constantina Bakolitsa; Connie Chen; Hsiu Ju Chiu; Michelle Chiu; Thomas Clayton; Marc C. Deller; Lian Duan; Kyle Ellrott; Marc André Elsliger; Dustin Ernst; Carol L. Farr; Julie Feuerhelm; Anna Grzechnik; Joanna C. Grant; Kevin K. Jin; Hope A. Johnson; Heath E. Klock; Mark W. Knuth; S. Sri Krishna; Abhinav Kumar; David Marciano; Daniel McMullan; Mitchell D. Miller; Andrew T. Morse; Edward Nigoghossian; Amanda Nopakun; Linda Okach; Silvya Oommachen; Jessica Paulsen; Christina Puckett; Ron Reyes; Christopher L. Rife; Natasha Sefcovic; Henry J. Tien; Christine B. Trame; Henry Van Den Bedem; Dana Weekes; Tiffany Wooten; Qingping Xu; Keith O. Hodgson; John Wooley; Ashley M. Deacon; Adam Godzik; Scott A. Lesley; Ian A. Wilson

doi:10.1107/S1744309109018168

The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold

Debanu Das, Piotr Kozbial, Gye Won Han, Dennis Carlton, Lukasz Jaroszewski, Polat Abdubek, Tamara Astakhova, Herbert L. Axelrod, Constantina Bakolitsa, Connie Chen, Hsiu Ju Chiu, Michelle Chiu, Thomas Clayton, Marc C. Deller, Lian Duan, Kyle Ellrott, Marc André Elsliger, Dustin Ernst, Carol L. Farr, Julie FeuerhelmAnna Grzechnik, Joanna C. Grant, Kevin K. Jin, Hope A. Johnson, Heath E. Klock, Mark W. Knuth, S. Sri Krishna, Abhinav Kumar, David Marciano, Daniel McMullan, Mitchell D. Miller, Andrew T. Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Silvya Oommachen, Jessica Paulsen, Christina Puckett, Ron Reyes, Christopher L. Rife, Natasha Sefcovic, Henry J. Tien, Christine B. Trame, Henry Van Den Bedem, Dana Weekes, Tiffany Wooten, Qingping Xu, Keith O. Hodgson, John Wooley, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Ian A. Wilson

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

KPN03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by Klebsiella pneumoniae MGH 78578. The crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the OB-fold and structurally similar to the bacterial Cpx-pathway protein NlpE, single-stranded DNA-binding (SSB) proteins and toxins. K. pneumoniae MGH 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pathogen that is linked to about 8% of all hospital-acquired infections in the USA. This structure provides the foundation for further investigations into this divergent member of the OB-fold family.

Original language	English (US)
Pages (from-to)	1254-1260
Number of pages	7
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	66
Issue number	10
DOIs	https://doi.org/10.1107/S1744309109018168
State	Published - Oct 2010
Externally published	Yes

Keywords

BOF
NipE-like protein
OB-fold
human gut pathogens
lipoproteins
single-stranded DNA-binding proteins
structural genomics
toxins

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S1744309109018168

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Das, D., Kozbial, P., Han, G. W., Carlton, D., Jaroszewski, L., Abdubek, P., Astakhova, T., Axelrod, H. L., Bakolitsa, C., Chen, C., Chiu, H. J., Chiu, M., Clayton, T., Deller, M. C., Duan, L., Ellrott, K., Elsliger, M. A., Ernst, D., Farr, C. L., ... Wilson, I. A. (2010). The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(10), 1254-1260. https://doi.org/10.1107/S1744309109018168

The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold. / Das, Debanu; Kozbial, Piotr; Han, Gye Won et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 10, 10.2010, p. 1254-1260.

Research output: Contribution to journal › Article › peer-review

Das, D, Kozbial, P, Han, GW, Carlton, D, Jaroszewski, L, Abdubek, P, Astakhova, T, Axelrod, HL, Bakolitsa, C, Chen, C, Chiu, HJ, Chiu, M, Clayton, T, Deller, MC, Duan, L, Ellrott, K, Elsliger, MA, Ernst, D, Farr, CL, Feuerhelm, J, Grzechnik, A, Grant, JC, Jin, KK, Johnson, HA, Klock, HE, Knuth, MW, Krishna, SS, Kumar, A, Marciano, D, McMullan, D, Miller, MD, Morse, AT, Nigoghossian, E, Nopakun, A, Okach, L, Oommachen, S, Paulsen, J, Puckett, C, Reyes, R, Rife, CL, Sefcovic, N, Tien, HJ, Trame, CB, Van Den Bedem, H, Weekes, D, Wooten, T, Xu, Q, Hodgson, KO, Wooley, J, Deacon, AM, Godzik, A, Lesley, SA & Wilson, IA 2010, 'The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 66, no. 10, pp. 1254-1260. https://doi.org/10.1107/S1744309109018168

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title = "The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold",

abstract = "KPN03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by Klebsiella pneumoniae MGH 78578. The crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the OB-fold and structurally similar to the bacterial Cpx-pathway protein NlpE, single-stranded DNA-binding (SSB) proteins and toxins. K. pneumoniae MGH 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pathogen that is linked to about 8% of all hospital-acquired infections in the USA. This structure provides the foundation for further investigations into this divergent member of the OB-fold family.",

keywords = "BOF, NipE-like protein, OB-fold, human gut pathogens, lipoproteins, single-stranded DNA-binding proteins, structural genomics, toxins",

author = "Debanu Das and Piotr Kozbial and Han, {Gye Won} and Dennis Carlton and Lukasz Jaroszewski and Polat Abdubek and Tamara Astakhova and Axelrod, {Herbert L.} and Constantina Bakolitsa and Connie Chen and Chiu, {Hsiu Ju} and Michelle Chiu and Thomas Clayton and Deller, {Marc C.} and Lian Duan and Kyle Ellrott and Elsliger, {Marc Andr{\'e}} and Dustin Ernst and Farr, {Carol L.} and Julie Feuerhelm and Anna Grzechnik and Grant, {Joanna C.} and Jin, {Kevin K.} and Johnson, {Hope A.} and Klock, {Heath E.} and Knuth, {Mark W.} and Krishna, {S. Sri} and Abhinav Kumar and David Marciano and Daniel McMullan and Miller, {Mitchell D.} and Morse, {Andrew T.} and Edward Nigoghossian and Amanda Nopakun and Linda Okach and Silvya Oommachen and Jessica Paulsen and Christina Puckett and Ron Reyes and Rife, {Christopher L.} and Natasha Sefcovic and Tien, {Henry J.} and Trame, {Christine B.} and {Van Den Bedem}, Henry and Dana Weekes and Tiffany Wooten and Qingping Xu and Hodgson, {Keith O.} and John Wooley and Deacon, {Ashley M.} and Adam Godzik and Lesley, {Scott A.} and Wilson, {Ian A.}",

year = "2010",

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doi = "10.1107/S1744309109018168",

language = "English (US)",

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pages = "1254--1260",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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AU - Han, Gye Won

AU - Carlton, Dennis

AU - Jaroszewski, Lukasz

AU - Abdubek, Polat

AU - Astakhova, Tamara

AU - Axelrod, Herbert L.

AU - Bakolitsa, Constantina

AU - Chen, Connie

AU - Chiu, Hsiu Ju

AU - Chiu, Michelle

AU - Clayton, Thomas

AU - Deller, Marc C.

AU - Duan, Lian

AU - Ellrott, Kyle

AU - Elsliger, Marc André

AU - Ernst, Dustin

AU - Farr, Carol L.

AU - Feuerhelm, Julie

AU - Grzechnik, Anna

AU - Grant, Joanna C.

AU - Jin, Kevin K.

AU - Johnson, Hope A.

AU - Klock, Heath E.

AU - Knuth, Mark W.

AU - Krishna, S. Sri

AU - Kumar, Abhinav

AU - Marciano, David

AU - McMullan, Daniel

AU - Miller, Mitchell D.

AU - Morse, Andrew T.

AU - Nigoghossian, Edward

AU - Nopakun, Amanda

AU - Okach, Linda

AU - Oommachen, Silvya

AU - Paulsen, Jessica

AU - Puckett, Christina

AU - Reyes, Ron

AU - Rife, Christopher L.

AU - Sefcovic, Natasha

AU - Tien, Henry J.

AU - Trame, Christine B.

AU - Van Den Bedem, Henry

AU - Weekes, Dana

AU - Wooten, Tiffany

AU - Xu, Qingping

AU - Hodgson, Keith O.

AU - Wooley, John

AU - Deacon, Ashley M.

AU - Godzik, Adam

AU - Lesley, Scott A.

AU - Wilson, Ian A.

PY - 2010/10

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KW - human gut pathogens

KW - lipoproteins

KW - single-stranded DNA-binding proteins

KW - structural genomics

KW - toxins

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The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold

Abstract

Keywords

ASJC Scopus subject areas

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