Abstract
The recent development of RNAi-based techniques for protein knockdown in mammalian cells has allowed for unprecedented flexibility in the study of protein function. Currently, large siRNA libraries are available that allow the knockdown of all proteins known to be encoded by the human genome. These libraries have been used to identify the host proteins required for the replication of several clinically important viruses, including HIV, flaviviruses and influenza. This review summarizes the methods used in RNAi-based screening for host factors involved in virus replication, and discusses published examples of such screens.
Original language | English (US) |
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Pages (from-to) | 303-311 |
Number of pages | 9 |
Journal | Future Microbiology |
Volume | 5 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2010 |
Keywords
- Flavivirus
- HIV
- Influenza
- Screen
- SiRNA
- Viral host factor
- Virus
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)