The yeast Snm1 protein is a DNA 5′-exonuclease

Xiaoroang Li; James Hejna; Robb E. Moses

doi:10.1016/j.dnarep.2004.08.012

The yeast Snm1 protein is a DNA 5′-exonuclease

Xiaoroang Li, James Hejna, Robb E. Moses

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Interstrand cross-links (ICL) in DNA arise from bifunctional alkylating agents, including nitrogen mustards, mitomycin C and psoralens. Such adducts prevent normal transcription or replication and are mutagenic. Therefore, cellular mechanisms for removing ICL damage are needed to maintain genome stability. Normal ICL repair requires the action of a number of genes, some specific for such damage. The yeast Snm1 protein is one such protein, but its function has been unknown. Incision for ICL repair is normal in mutants lacking Snm1, so it appears to act after the earliest steps. We have used recombinant SNM1 constructs in an Escherichia coli (E. coli) expression system to demonstrate that the yeast gene encodes a 5′-exonuclease. The exonuclease activity is required for Snm1 to be functional in ICL repair.

Original language	English (US)
Pages (from-to)	163-170
Number of pages	8
Journal	DNA Repair
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/j.dnarep.2004.08.012
State	Published - Feb 3 2005
Externally published	Yes

Keywords

DNA Repair
Exonuclease
Interstrand cross-links
SNM1

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.dnarep.2004.08.012

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title = "The yeast Snm1 protein is a DNA 5′-exonuclease",

abstract = "Interstrand cross-links (ICL) in DNA arise from bifunctional alkylating agents, including nitrogen mustards, mitomycin C and psoralens. Such adducts prevent normal transcription or replication and are mutagenic. Therefore, cellular mechanisms for removing ICL damage are needed to maintain genome stability. Normal ICL repair requires the action of a number of genes, some specific for such damage. The yeast Snm1 protein is one such protein, but its function has been unknown. Incision for ICL repair is normal in mutants lacking Snm1, so it appears to act after the earliest steps. We have used recombinant SNM1 constructs in an Escherichia coli (E. coli) expression system to demonstrate that the yeast gene encodes a 5′-exonuclease. The exonuclease activity is required for Snm1 to be functional in ICL repair.",

keywords = "DNA Repair, Exonuclease, Interstrand cross-links, SNM1",

author = "Xiaoroang Li and James Hejna and Moses, {Robb E.}",

note = "Funding Information: This work was supported by grant P01-HL48546 from the United States National Institutes of Health. We thank M. Matkovic for materials and advice.",

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T1 - The yeast Snm1 protein is a DNA 5′-exonuclease

AU - Li, Xiaoroang

AU - Hejna, James

AU - Moses, Robb E.

N1 - Funding Information: This work was supported by grant P01-HL48546 from the United States National Institutes of Health. We thank M. Matkovic for materials and advice.

PY - 2005/2/3

Y1 - 2005/2/3

N2 - Interstrand cross-links (ICL) in DNA arise from bifunctional alkylating agents, including nitrogen mustards, mitomycin C and psoralens. Such adducts prevent normal transcription or replication and are mutagenic. Therefore, cellular mechanisms for removing ICL damage are needed to maintain genome stability. Normal ICL repair requires the action of a number of genes, some specific for such damage. The yeast Snm1 protein is one such protein, but its function has been unknown. Incision for ICL repair is normal in mutants lacking Snm1, so it appears to act after the earliest steps. We have used recombinant SNM1 constructs in an Escherichia coli (E. coli) expression system to demonstrate that the yeast gene encodes a 5′-exonuclease. The exonuclease activity is required for Snm1 to be functional in ICL repair.

AB - Interstrand cross-links (ICL) in DNA arise from bifunctional alkylating agents, including nitrogen mustards, mitomycin C and psoralens. Such adducts prevent normal transcription or replication and are mutagenic. Therefore, cellular mechanisms for removing ICL damage are needed to maintain genome stability. Normal ICL repair requires the action of a number of genes, some specific for such damage. The yeast Snm1 protein is one such protein, but its function has been unknown. Incision for ICL repair is normal in mutants lacking Snm1, so it appears to act after the earliest steps. We have used recombinant SNM1 constructs in an Escherichia coli (E. coli) expression system to demonstrate that the yeast gene encodes a 5′-exonuclease. The exonuclease activity is required for Snm1 to be functional in ICL repair.

KW - DNA Repair

KW - Exonuclease

KW - Interstrand cross-links

KW - SNM1

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The yeast Snm1 protein is a DNA 5′-exonuclease

Abstract

Keywords

ASJC Scopus subject areas

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