TY - JOUR
T1 - Three-dimensional reconstruction from serial sections in PC-Windows platform by using 3D_Viewer
AU - Xu, Yi Hua
AU - Lahvis, Garet
AU - Edwards, Harlene
AU - Pitot, Henry C.
N1 - Funding Information:
The authors express their sincere appreciation to Mrs. Mary Jo Markham and Mrs. Kristen Adler for their expert technical typing of the manuscript and to Dr. Ilse Riegel for her critical editorial comments. The development of this software was supported in part by grants from the National Cancer Institute of the United States (CA07175, CA22484, and CA45700).
PY - 2004/11
Y1 - 2004/11
N2 - Three-dimensional (3D) reconstruction from serial sections allows identification of objects of interest in 3D and clarifies the relationship among these objects. 3D_Viewer, developed in our laboratory for this purpose, has four major functions: image alignment, movie frame production, movie viewing, and shift-overlay image generation. Color images captured from serial sections were aligned; then the contours of objects of interest were highlighted in a semi-automatic manner. These 2D images were then automatically stacked at different viewing angles, and their composite images on a projected plane were recorded by an image transform-shift-overlay technique. These composition images are used in the object-rotation movie show. The design considerations of the program and the procedures used for 3D reconstruction from serial sections are described. This program, with a digital image-capture system, a semi-automatic contours highlight method, and an automatic image transform-shift-overlay technique, greatly speeds up the reconstruction process. Since images generated by 3D_Viewer are in a general graphic format, data sharing with others is easy. 3D_Viewer is written in MS Visual Basic 6, obtainable from our laboratory on request.
AB - Three-dimensional (3D) reconstruction from serial sections allows identification of objects of interest in 3D and clarifies the relationship among these objects. 3D_Viewer, developed in our laboratory for this purpose, has four major functions: image alignment, movie frame production, movie viewing, and shift-overlay image generation. Color images captured from serial sections were aligned; then the contours of objects of interest were highlighted in a semi-automatic manner. These 2D images were then automatically stacked at different viewing angles, and their composite images on a projected plane were recorded by an image transform-shift-overlay technique. These composition images are used in the object-rotation movie show. The design considerations of the program and the procedures used for 3D reconstruction from serial sections are described. This program, with a digital image-capture system, a semi-automatic contours highlight method, and an automatic image transform-shift-overlay technique, greatly speeds up the reconstruction process. Since images generated by 3D_Viewer are in a general graphic format, data sharing with others is easy. 3D_Viewer is written in MS Visual Basic 6, obtainable from our laboratory on request.
KW - 3D_Viewer
KW - Image align
KW - Image transform-shift-overlay technique
KW - MS Visual Basic
KW - Movie-frame-image
KW - PC_Windows
KW - Three-dimensional reconstruction
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U2 - 10.1016/j.cmpb.2004.04.002
DO - 10.1016/j.cmpb.2004.04.002
M3 - Article
C2 - 15451163
AN - SCOPUS:4644360343
SN - 0169-2607
VL - 76
SP - 143
EP - 154
JO - Computer Methods and Programs in Biomedicine
JF - Computer Methods and Programs in Biomedicine
IS - 2
ER -