Titration of a vaccine stock preparation of human immunodeficiency virus type 1(SF2) in cultured lymphocytes and in chimpanzees

Krishna K. Murthy; E. Kathy Cobb; Zahwa El-Amad; Henry Ortega; Frank C. Hsueh; William Satterfield; D. Rick Lee; Marcia L. Kalish; Nancy L. Haigwood; Ronald C. Kennedy; Kathelyn S. Steimer; Alan Schultz; Jay A. Levy

doi:10.1089/aid.1996.12.1341

Titration of a vaccine stock preparation of human immunodeficiency virus type 1(SF2) in cultured lymphocytes and in chimpanzees

Krishna K. Murthy, E. Kathy Cobb, Zahwa El-Amad, Henry Ortega, Frank C. Hsueh, William Satterfield, D. Rick Lee, Marcia L. Kalish, Nancy L. Haigwood, Ronald C. Kennedy, Kathelyn S. Steimer, Alan Schultz, Jay A. Levy

Research output: Contribution to journal › Article › peer-review

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Abstract

A large stock preparation of the HIV-1(SF2) isolate has been derived after serial passage in human peripheral blood mononuclear cells (PBMCs). This viral stock has a titer of 10^4.9 TCID₅₀ in human PBMCs and 10^4.2 TCID₅₀ in chimpanzee PBMCs. By inoculation into animals the 50% chimpanzee infections dose titer was found to be about 10^2.3. Virus isolation from animals was achieved on most occasions within 1-4 weeks after inoculation and then became transient. Viral RNA and DNA PCR analyses confirmed the virus infection of the chimpanzees. Anti-HIV antibody levels in the inoculated animals ranged from 1:400 to 1:6400 as measured by ELISA. About 680 vials of this stock preparation, frozen at -190°C, are available for future studies of vaccines and antiviral therapies.

Original language	English (US)
Pages (from-to)	1341-1348
Number of pages	8
Journal	AIDS Research and Human Retroviruses
Volume	12
Issue number	14
DOIs	https://doi.org/10.1089/aid.1996.12.1341
State	Published - Sep 20 1996
Externally published	Yes

ASJC Scopus subject areas

Infectious Diseases
Virology
Immunology

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Murthy, K. K., Cobb, E. K., El-Amad, Z., Ortega, H., Hsueh, F. C., Satterfield, W., Lee, D. R., Kalish, M. L., Haigwood, N. L., Kennedy, R. C., Steimer, K. S., Schultz, A., & Levy, J. A. (1996). Titration of a vaccine stock preparation of human immunodeficiency virus type 1(SF2) in cultured lymphocytes and in chimpanzees. AIDS Research and Human Retroviruses, 12(14), 1341-1348. https://doi.org/10.1089/aid.1996.12.1341

Murthy, KK, Cobb, EK, El-Amad, Z, Ortega, H, Hsueh, FC, Satterfield, W, Lee, DR, Kalish, ML, Haigwood, NL, Kennedy, RC, Steimer, KS, Schultz, A & Levy, JA 1996, 'Titration of a vaccine stock preparation of human immunodeficiency virus type 1(SF2) in cultured lymphocytes and in chimpanzees', AIDS Research and Human Retroviruses, vol. 12, no. 14, pp. 1341-1348. https://doi.org/10.1089/aid.1996.12.1341

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abstract = "A large stock preparation of the HIV-1(SF2) isolate has been derived after serial passage in human peripheral blood mononuclear cells (PBMCs). This viral stock has a titer of 104.9 TCID50 in human PBMCs and 104.2 TCID50 in chimpanzee PBMCs. By inoculation into animals the 50% chimpanzee infections dose titer was found to be about 102.3. Virus isolation from animals was achieved on most occasions within 1-4 weeks after inoculation and then became transient. Viral RNA and DNA PCR analyses confirmed the virus infection of the chimpanzees. Anti-HIV antibody levels in the inoculated animals ranged from 1:400 to 1:6400 as measured by ELISA. About 680 vials of this stock preparation, frozen at -190°C, are available for future studies of vaccines and antiviral therapies.",

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AU - Ortega, Henry

AU - Hsueh, Frank C.

AU - Satterfield, William

AU - Lee, D. Rick

AU - Kalish, Marcia L.

AU - Haigwood, Nancy L.

AU - Kennedy, Ronald C.

AU - Steimer, Kathelyn S.

AU - Schultz, Alan

AU - Levy, Jay A.

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Titration of a vaccine stock preparation of human immunodeficiency virus type 1(SF2) in cultured lymphocytes and in chimpanzees

Abstract

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