Abstract
We have previously demonstrated that mycobacterial lipoproteins engage TLR2 on human CD4+ T cells and upregulate TCR-triggered IFN-γ secretion and cell proliferation in vitro. Here we examined the role of CD4+ T-cell-expressed TLR2 in Mycobacterium tuberculosis (MTB) Ag-specific T-cell priming and in protection against MTB infection in vivo. Like their human counterparts, mouse CD4+ T cells express TLR2 and respond to TLR2 costimulation in vitro. This Th1-like response was observed in the context of both polyclonal and Ag-specific TCR stimulation. To evaluate the role of T-cell TLR2 in priming of CD4+ T cells in vivo, naive MTB Ag85B-specific TCR transgenic CD4+ T cells (P25 TCR-Tg) were adoptively transferred into Tlr2-/- recipient C57BL/6 mice that were then immunized with Ag85B and with or without TLR2 ligand Pam3Cys-SKKKK. TLR2 engagement during priming resulted in increased numbers of IFN-γ-secreting P25 TCR-Tg T cells 1 week after immunization. P25 TCR-Tg T cells stimulated in vitro via TCR and TLR2 conferred more protection than T cells stimulated via TCR alone when adoptively transferred before MTB infection. Our findings indicate that TLR2 engagement on CD4+ T cells increases MTB Ag-specific responses and may contribute to protection against MTB infection.
Original language | English (US) |
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Pages (from-to) | 1410-1421 |
Number of pages | 12 |
Journal | European Journal of Immunology |
Volume | 44 |
Issue number | 5 |
DOIs | |
State | Published - May 2014 |
Keywords
- Mycobacterium tuberculosis
- Toll-like receptor 2
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology