Transcriptional mapping of Leishmania enriettii tubulin mRNAs

Scott M. Landfear, Samuel I. Miller, Dyann F. Wirth

Research output: Contribution to journalArticlepeer-review


We have mapped the mRNAs for α- and β-tubulins of Leishmania enrietti promastigotes and amastigotes and have demonstrated that each RNA contains a 35 nucleotide sequence on its 5′ end which is not encoded contiguously with the rest of the message. Sequencing of the 5′ end of the α- and β-tubulin mRNAs revealed that this 35 nucleotide leader sequence is identical in both messages and that it is homologous to the spliced leader found on the 5′ end of mRNAs in the related parasite Trypanosoma brucei. Additionally, we have sequenced regions of the α- and β-tubulin genomic clones upstream from the mRNA encoding regions and have shown that the leader sequence is not encoded within these regions of DNA. Hence, Leishmania tubulin mRNAs may be synthesized by a discontinuous transcription process that links together the transcription products of two separate gene families, as suggested previously by others for the assembly of T. brucei mRNAs. Homologies between the Leishmania α- and β-tubulin genes themselves and between these genes and the T. brucei α- and β-tubulin genes have revealed sequences which may be important in synthesis or processing of Leishmania tubulin mRNAs.

Original languageEnglish (US)
Pages (from-to)235-245
Number of pages11
JournalMolecular and Biochemical Parasitology
Issue number3
StatePublished - Dec 1986
Externally publishedYes


  • Leishmania
  • Transcription mapping
  • Tubulin genes
  • mRNA leader sequence

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology


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