TY - JOUR
T1 - Transcriptomic and epigenomic characterization of the developing bat wing
AU - Eckalbar, Walter L.
AU - Schlebusch, Stephen A.
AU - Mason, Mandy K.
AU - Gill, Zoe
AU - Parker, Ash V.
AU - Booker, Betty M.
AU - Nishizaki, Sierra
AU - Muswamba-Nday, Christiane
AU - Terhune, Elizabeth
AU - Nevonen, Kimberly A.
AU - Makki, Nadja
AU - Friedrich, Tara
AU - VanderMeer, Julia E.
AU - Pollard, Katherine S.
AU - Carbone, Lucia
AU - Wall, Jeff D.
AU - Illing, Nicola
AU - Ahituv, Nadav
N1 - Publisher Copyright:
© 2016 Nature America, Inc.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Bats are the only mammals capable of powered flight, but little is known about the genetic determinants that shape their wings. Here we generated a genome for Miniopterus natalensis and performed RNA-seq and ChIP-seq (H3K27ac and H3K27me3) analyses on its developing forelimb and hindlimb autopods at sequential embryonic stages to decipher the molecular events that underlie bat wing development. Over 7,000 genes and several long noncoding RNAs, including Tbx5-as1 and Hottip, were differentially expressed between forelimb and hindlimb, and across different stages. ChIP-seq analysis identified thousands of regions that are differentially modified in forelimb and hindlimb. Comparative genomics found 2,796 bat-accelerated regions within H3K27ac peaks, several of which cluster near limb-associated genes. Pathway analyses highlighted multiple ribosomal proteins and known limb patterning signaling pathways as differentially regulated and implicated increased forelimb mesenchymal condensation in differential growth. In combination, our work outlines multiple genetic components that likely contribute to bat wing formation, providing insights into this morphological innovation.
AB - Bats are the only mammals capable of powered flight, but little is known about the genetic determinants that shape their wings. Here we generated a genome for Miniopterus natalensis and performed RNA-seq and ChIP-seq (H3K27ac and H3K27me3) analyses on its developing forelimb and hindlimb autopods at sequential embryonic stages to decipher the molecular events that underlie bat wing development. Over 7,000 genes and several long noncoding RNAs, including Tbx5-as1 and Hottip, were differentially expressed between forelimb and hindlimb, and across different stages. ChIP-seq analysis identified thousands of regions that are differentially modified in forelimb and hindlimb. Comparative genomics found 2,796 bat-accelerated regions within H3K27ac peaks, several of which cluster near limb-associated genes. Pathway analyses highlighted multiple ribosomal proteins and known limb patterning signaling pathways as differentially regulated and implicated increased forelimb mesenchymal condensation in differential growth. In combination, our work outlines multiple genetic components that likely contribute to bat wing formation, providing insights into this morphological innovation.
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U2 - 10.1038/ng.3537
DO - 10.1038/ng.3537
M3 - Article
C2 - 27019111
AN - SCOPUS:84961922811
SN - 1061-4036
VL - 48
SP - 528
EP - 536
JO - Nature genetics
JF - Nature genetics
IS - 5
ER -