Transient nature of interference effects from heterophile antibodies: Examples of interference with cardiac marker measurements

Steven C. Kazmierczak, Paul G. Catrou, Kimberly P. Briley

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Two-site immunoassay methods have become the standard technique for measurement of a wide variety of drugs, hormones, and cell proteins. One limitation of these methods is their susceptibility to interference from heterophilic antibodies present in the sera of some patients. Human anti-murine antibodies represent a common heterophile antibody that can bind to mouse immunoglobulin and as well as to immunoglobulin from other species. While the mechanism of human anti-murine antibody interference has been well characterized, the time course over which this interference occurs and the susceptibility of different immunoassay procedures to human anti-murine antibody interference from patients with human anti-murine antibody have not been as well described. We report on the time course of interference in assays for cardiac markers for two patients with human anti-murine antibodies. We measured creatine kinase MB isoenzyme (CKMB) and troponins I and T using three different vendors' immunoassay procedures. Our results demonstrate that assay interference due to human anti-murine antibody interference is a transient phenomenon. In one of our patients, human anti-murine antibody interference appeared suddenly, peaked approximately 9 days following its appearance, and gradually resolved over the next 3 weeks. In addition, we found that immunoassay methods from different vendors can show highly variable interference effects when human anti-murine antibody-containing specimens are analyzed.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Issue number1
StatePublished - 2000
Externally publishedYes


  • Cardiac marker measurement
  • Human anti-murine antibodies
  • Interference

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical


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