Trauma and alkali burns induce distinct patterns of cytokine gene expression in the rat cornea

S. R. Planck; L. F. Rich; J. C. Ansel; X. N. Huang; J. T. Rosenbaum

doi:10.3109/09273949709085057

Trauma and alkali burns induce distinct patterns of cytokine gene expression in the rat cornea

S. R. Planck
, L. F. Rich
, J. C. Ansel
, X. N. Huang
, J. T. Rosenbaum

Ophthalmology

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Cytokines such as the interleukins (IL) and tumor necrosis factor alpha (TNFα) have traditionally been associated with paracrine regulation of immune reactions. These proteins also have properties suggestive of functional roles in the inflammatory and reparative responses to tissue injury. In this study, mRNA levels for IL-1α, IL-1β, IL-6, TNFα, interferon γ, transforming growth factor B₁ and CD₄ were monitored in rat corneas at times from 1 hour through 2 weeks after incisional trauma of alkali burns. Transcripts for IL-1α, TNFα, and TGFβ₁, were present in most corneal samples; whereas those for IFNγ and CD₄ were not detected. As early as 1 hour following either of these non-immunologic forms of injury, expression of IL-6 mRNA levels was induced. Only in corneas with alkali burns did IL-6 induction persist from days 1 through 7. The alkali-injured corneas also had markedly increased IL-1β mRNA levels from days 1 through 7. These observations indicate that cytokine mRNA is induced in the cornea by trauma without an apparent immunologic stimulus. Our data are consistent with the hypothesis that corneal tissues respond to different types of injury with different patterns of cytokine gene expression.

Original language	English (US)
Pages (from-to)	95-100
Number of pages	6
Journal	Ocular Immunology and Inflammation
Volume	5
Issue number	2
DOIs	https://doi.org/10.3109/09273949709085057
State	Published - Jan 1 1997

Funding

Stephen Planck, Ph.D., Casey Eye Institute, Oregon Health Sciences University, 3375 SW Terwilliger Blvd., Portland, OR 97201, USA. Phone: (503) 494-51 10; fax: (503) 494-6875; e-mail: [email protected] Acknowledgment: Research was supported by NIH grants EY06484, EY06477, EY07373. Additional support was obtained from a Senior Scholar Award (JTR), a Special Scholar Award (SRP), and an unrestricted grant from Research to Prevent Blindness.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	EY06477, EY06484
National Eye Institute and Casey Eye Institute	R01EY007373
Research to Prevent Blindness

Keywords

Cornea
Interleukin-1
Interleukin-6
Wound healing

ASJC Scopus subject areas

Immunology and Allergy
Ophthalmology

Access to Document

10.3109/09273949709085057

Cite this

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title = "Trauma and alkali burns induce distinct patterns of cytokine gene expression in the rat cornea",

abstract = "Cytokines such as the interleukins (IL) and tumor necrosis factor alpha (TNFα) have traditionally been associated with paracrine regulation of immune reactions. These proteins also have properties suggestive of functional roles in the inflammatory and reparative responses to tissue injury. In this study, mRNA levels for IL-1α, IL-1β, IL-6, TNFα, interferon γ, transforming growth factor B1 and CD4 were monitored in rat corneas at times from 1 hour through 2 weeks after incisional trauma of alkali burns. Transcripts for IL-1α, TNFα, and TGFβ1, were present in most corneal samples; whereas those for IFNγ and CD4 were not detected. As early as 1 hour following either of these non-immunologic forms of injury, expression of IL-6 mRNA levels was induced. Only in corneas with alkali burns did IL-6 induction persist from days 1 through 7. The alkali-injured corneas also had markedly increased IL-1β mRNA levels from days 1 through 7. These observations indicate that cytokine mRNA is induced in the cornea by trauma without an apparent immunologic stimulus. Our data are consistent with the hypothesis that corneal tissues respond to different types of injury with different patterns of cytokine gene expression.",

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note = "Funding Information: Stephen Planck, Ph.D., Casey Eye Institute, Oregon Health Sciences University, 3375 SW Terwilliger Blvd., Portland, OR 97201, USA. Phone: (503) 494-51 10; fax: (503) 494-6875; e-mail: [email protected] Acknowledgment: Research was supported by NIH grants EY06484, EY06477, EY07373. Additional support was obtained from a Senior Scholar Award (JTR), a Special Scholar Award (SRP), and an unrestricted grant from Research to Prevent Blindness.",

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AB - Cytokines such as the interleukins (IL) and tumor necrosis factor alpha (TNFα) have traditionally been associated with paracrine regulation of immune reactions. These proteins also have properties suggestive of functional roles in the inflammatory and reparative responses to tissue injury. In this study, mRNA levels for IL-1α, IL-1β, IL-6, TNFα, interferon γ, transforming growth factor B1 and CD4 were monitored in rat corneas at times from 1 hour through 2 weeks after incisional trauma of alkali burns. Transcripts for IL-1α, TNFα, and TGFβ1, were present in most corneal samples; whereas those for IFNγ and CD4 were not detected. As early as 1 hour following either of these non-immunologic forms of injury, expression of IL-6 mRNA levels was induced. Only in corneas with alkali burns did IL-6 induction persist from days 1 through 7. The alkali-injured corneas also had markedly increased IL-1β mRNA levels from days 1 through 7. These observations indicate that cytokine mRNA is induced in the cornea by trauma without an apparent immunologic stimulus. Our data are consistent with the hypothesis that corneal tissues respond to different types of injury with different patterns of cytokine gene expression.

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Trauma and alkali burns induce distinct patterns of cytokine gene expression in the rat cornea

Abstract

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Keywords

ASJC Scopus subject areas

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