Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions

Anna Bar; Nicholas Snavely; Nathaniel Chen; Steven Jacques; Daniel S. Gareau

doi:10.1117/12.909902

Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions

Anna Bar, Nicholas Snavely, Nathaniel Chen, Steven Jacques, Daniel S. Gareau

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Screening cancer in excision margins may be done with confocal microscopy to save time and cost over the gold standard histopathology (H&E). However, diagnostic accuracy requires sufficient contrast. Reflectance mode enables detection of large (>500um) nodular tumors. Enhanced nuclear contrast with acridine orange fluorescence mode additionally enables detection of tiny (<50um) basal cell carcinomas. Here, we present a novel combination of three modes to detect squamous cell carcinoma (SCC). Accurate screening of SCC requires eosin fluorescence, reflectance and acridine orange fluorescence to enable contrast for cytoplasm, collagen and nuclei respectively. Combining these signals replicates H&E for rapid clinical translation.

Original language	English (US)
Title of host publication	Advanced Biomedical and Clinical Diagnostic Systems X
DOIs	https://doi.org/10.1117/12.909902
State	Published - 2012
Event	Advanced Biomedical and Clinical Diagnostic Systems X - San Francisco, CA, United States Duration: Jan 22 2012 → Jan 24 2012

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8214
ISSN (Print)	1605-7422

Other

Other	Advanced Biomedical and Clinical Diagnostic Systems X
Country/Territory	United States
City	San Francisco, CA
Period	1/22/12 → 1/24/12

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging
Biomaterials

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10.1117/12.909902

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Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions. / Bar, Anna; Snavely, Nicholas; Chen, Nathaniel et al.
Advanced Biomedical and Clinical Diagnostic Systems X. 2012. 821416 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8214).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Bar, A, Snavely, N, Chen, N, Jacques, S & Gareau, DS 2012, Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions. in Advanced Biomedical and Clinical Diagnostic Systems X., 821416, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8214, Advanced Biomedical and Clinical Diagnostic Systems X, San Francisco, CA, United States, 1/22/12. https://doi.org/10.1117/12.909902

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abstract = "Screening cancer in excision margins may be done with confocal microscopy to save time and cost over the gold standard histopathology (H&E). However, diagnostic accuracy requires sufficient contrast. Reflectance mode enables detection of large (>500um) nodular tumors. Enhanced nuclear contrast with acridine orange fluorescence mode additionally enables detection of tiny (<50um) basal cell carcinomas. Here, we present a novel combination of three modes to detect squamous cell carcinoma (SCC). Accurate screening of SCC requires eosin fluorescence, reflectance and acridine orange fluorescence to enable contrast for cytoplasm, collagen and nuclei respectively. Combining these signals replicates H&E for rapid clinical translation.",

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PY - 2012

Y1 - 2012

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AB - Screening cancer in excision margins may be done with confocal microscopy to save time and cost over the gold standard histopathology (H&E). However, diagnostic accuracy requires sufficient contrast. Reflectance mode enables detection of large (>500um) nodular tumors. Enhanced nuclear contrast with acridine orange fluorescence mode additionally enables detection of tiny (<50um) basal cell carcinomas. Here, we present a novel combination of three modes to detect squamous cell carcinoma (SCC). Accurate screening of SCC requires eosin fluorescence, reflectance and acridine orange fluorescence to enable contrast for cytoplasm, collagen and nuclei respectively. Combining these signals replicates H&E for rapid clinical translation.

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M3 - Conference contribution

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Advanced Biomedical and Clinical Diagnostic Systems X

T2 - Advanced Biomedical and Clinical Diagnostic Systems X

Y2 - 22 January 2012 through 24 January 2012

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Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions

Abstract

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