TY - JOUR
T1 - Two distinct mechanisms target membrane proteins to the axonal surface
AU - Sampo, Bernard
AU - Kaech, Stefanie
AU - Kunz, Stefan
AU - Banker, Gary
N1 - Funding Information:
This research was supported by NIH grants NS17112 and MH66179. We are particularly grateful to Caroline Kingman and Barbara Smoody for their expert technical assistance. Peter Sonderegger kindly provided antibodies, constructs, and helpful advice.
PY - 2003/2/20
Y1 - 2003/2/20
N2 - We have investigated the trafficking of two endogenous axonal membrane proteins, VAMP2 and NgCAM, in order to elucidate the cellular events that underlie their polarization. We found that VAMP2 is delivered to the surface of both axons and dendrites, but preferentially endocytosed from the dendritic membrane. A mutation in the cytoplasmic domain of VAMP2 that inhibits endocytosis abolished its axonal polarization. In contrast, the targeting of NgCAM depends on sequences in its ectodomain, which mediate its sorting into carriers that preferentially deliver their cargo proteins to the axonal membrane. These observations show that neurons use two distinct mechanisms to polarize proteins to the axonal domain: selective retention in the case of VAMP2, selective delivery in the case of NgCAM.
AB - We have investigated the trafficking of two endogenous axonal membrane proteins, VAMP2 and NgCAM, in order to elucidate the cellular events that underlie their polarization. We found that VAMP2 is delivered to the surface of both axons and dendrites, but preferentially endocytosed from the dendritic membrane. A mutation in the cytoplasmic domain of VAMP2 that inhibits endocytosis abolished its axonal polarization. In contrast, the targeting of NgCAM depends on sequences in its ectodomain, which mediate its sorting into carriers that preferentially deliver their cargo proteins to the axonal membrane. These observations show that neurons use two distinct mechanisms to polarize proteins to the axonal domain: selective retention in the case of VAMP2, selective delivery in the case of NgCAM.
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U2 - 10.1016/S0896-6273(03)00058-8
DO - 10.1016/S0896-6273(03)00058-8
M3 - Article
C2 - 12597859
AN - SCOPUS:0037456539
SN - 0896-6273
VL - 37
SP - 611
EP - 624
JO - Neuron
JF - Neuron
IS - 4
ER -