Two ribeye genes in teleosts: The role of ribeye in ribbon formation and bipolar cell development

Lei Wan, Wolfhard Almers, Wenbiao Chen

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Ribeye is the only known protein specific to synaptic ribbon, but its function is unclear. We show that the teleost fish, Fugu and zebrafish, have two ribeye genes, ribeye a and ribeye b. Whole-mount in situ hybridization revealed that ribeye a is expressed in tissues containing synaptic ribbons, including the pineal gland, inner ear, and retina. Ribeye b is absent in the pineal gland. In the retina, ribeye a is expressed in both photoreceptors and bipolar cells, whereas ribeye b is detected only in photoreceptors. To study the function of Ribeye a in retina, we depleted it by morpholino antisense oligos. Fish deficient in Ribeye a lack an optokinetic response and have shorter synaptic ribbons in photoreceptors and fewer synaptic ribbons in bipolar cells. Their bipolar cells still target Syntaxin-3 proteins to the inner plexiform layer and have abundant vsx1 mRNA. However, they lack large synaptic terminals and show increased apoptosis. Rod bipolar cells are fewer in number and/or deficient in PKCα. Recovery of Ribeye a levels rescues the optokinetic response, increases the number of PKCα-positive bipolar cells, and stops apoptosis. We conclude that Ribeye a is important for late steps in bipolar cell development.

Original languageEnglish (US)
Pages (from-to)941-949
Number of pages9
JournalJournal of Neuroscience
Issue number4
StatePublished - Feb 26 2005


  • Apoptosis
  • Bipolar cells
  • Morpholino antisense oligo
  • Optokinetic response
  • Retina
  • Ribeye
  • Synaptic ribbon
  • Synaptogenesis

ASJC Scopus subject areas

  • General Neuroscience


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