Unfolded Protein Response Genes Regulated by CED-1 Are Required for Caenorhabditis elegans Innate Immunity

Kylie A. Haskins; Jonathan F. Russell; Nathan Gaddis; Holly K. Dressman; Alejandro Aballay

doi:10.1016/j.devcel.2008.05.006

Unfolded Protein Response Genes Regulated by CED-1 Are Required for Caenorhabditis elegans Innate Immunity

Kylie A. Haskins, Jonathan F. Russell, Nathan Gaddis, Holly K. Dressman, Alejandro Aballay

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

The endoplasmic reticulum stress response, also known as the unfolded protein response (UPR), has been implicated in the normal physiology of immune defense and in several disorders, including diabetes, cancer, and neurodegenerative disease. Here, we show that the apoptotic receptor CED-1 and a network of PQN/ABU proteins involved in a noncanonical UPR response are required for proper defense to pathogen infection in Caenorhabditis elegans. A full-genome microarray analysis indicates that CED-1 functions to activate the expression of pqn/abu genes. We also show that ced-1 and pqn/abu genes are required for the survival of C. elegans exposed to live Salmonella enterica, and that overexpression of pqn/abu genes confers protection against pathogen-mediated killing. The results indicate that unfolded protein response genes, regulated in a CED-1-dependent manner, are involved in the C. elegans immune response to live bacteria.

Original language	English (US)
Pages (from-to)	87-97
Number of pages	11
Journal	Developmental Cell
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/j.devcel.2008.05.006
State	Published - Jul 8 2008
Externally published	Yes

Keywords

CELLBIO
MOLIMMUNO

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2008.05.006

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title = "Unfolded Protein Response Genes Regulated by CED-1 Are Required for Caenorhabditis elegans Innate Immunity",

abstract = "The endoplasmic reticulum stress response, also known as the unfolded protein response (UPR), has been implicated in the normal physiology of immune defense and in several disorders, including diabetes, cancer, and neurodegenerative disease. Here, we show that the apoptotic receptor CED-1 and a network of PQN/ABU proteins involved in a noncanonical UPR response are required for proper defense to pathogen infection in Caenorhabditis elegans. A full-genome microarray analysis indicates that CED-1 functions to activate the expression of pqn/abu genes. We also show that ced-1 and pqn/abu genes are required for the survival of C. elegans exposed to live Salmonella enterica, and that overexpression of pqn/abu genes confers protection against pathogen-mediated killing. The results indicate that unfolded protein response genes, regulated in a CED-1-dependent manner, are involved in the C. elegans immune response to live bacteria.",

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author = "Haskins, {Kylie A.} and Russell, {Jonathan F.} and Nathan Gaddis and Dressman, {Holly K.} and Alejandro Aballay",

note = "Funding Information: We thank the Caenorhabditis Genetics Center (University of Minnesota) for providing all mutant strains used in this study. We also thank the Ron and Guarente laboratories for generously providing the abu-1 and abu-11 transgenic strains, respectively. Jennifer Tenor provided technical advice and performed the initial pharyngeal invasion assays. Holly K. Dressman provided excellent technical advice on microarray data analysis. A.A. is funded by The Whitehead Scholars Program, National Institutes of Health (NIH) Southeast Regional Center of Excellence for Emerging Infections and Biodefense (U54 AI057157), and NIH GM070977. ",

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AU - Dressman, Holly K.

AU - Aballay, Alejandro

N1 - Funding Information: We thank the Caenorhabditis Genetics Center (University of Minnesota) for providing all mutant strains used in this study. We also thank the Ron and Guarente laboratories for generously providing the abu-1 and abu-11 transgenic strains, respectively. Jennifer Tenor provided technical advice and performed the initial pharyngeal invasion assays. Holly K. Dressman provided excellent technical advice on microarray data analysis. A.A. is funded by The Whitehead Scholars Program, National Institutes of Health (NIH) Southeast Regional Center of Excellence for Emerging Infections and Biodefense (U54 AI057157), and NIH GM070977.

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N2 - The endoplasmic reticulum stress response, also known as the unfolded protein response (UPR), has been implicated in the normal physiology of immune defense and in several disorders, including diabetes, cancer, and neurodegenerative disease. Here, we show that the apoptotic receptor CED-1 and a network of PQN/ABU proteins involved in a noncanonical UPR response are required for proper defense to pathogen infection in Caenorhabditis elegans. A full-genome microarray analysis indicates that CED-1 functions to activate the expression of pqn/abu genes. We also show that ced-1 and pqn/abu genes are required for the survival of C. elegans exposed to live Salmonella enterica, and that overexpression of pqn/abu genes confers protection against pathogen-mediated killing. The results indicate that unfolded protein response genes, regulated in a CED-1-dependent manner, are involved in the C. elegans immune response to live bacteria.

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Unfolded Protein Response Genes Regulated by CED-1 Are Required for Caenorhabditis elegans Innate Immunity

Abstract

Keywords

ASJC Scopus subject areas

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