TY - JOUR
T1 - Vasoactive intestinal peptide activates Ca2+ -dependent K+ channels through a cAMP pathway in mouse lacrimal cells
AU - Lechleiter, James D.
AU - Dartt, Darlene A.
AU - Brehm, Paul
N1 - Funding Information:
This work was supported by NIH fellowship EY05824 to (J. D. L.) and NIH grants EY06177 (to D. A. D.) and NS18205 (to P 5.). We thank Drs. Patricia Camacho, Stan Rane, and Kathy Dunlap for their critical reading of this manuscript and their thoughtful suggestions. CAMPdependent protein kinase was a gift of Dr. Ray Erickson. Charybdotoxin was a gift of Dr. Chris Miller.
PY - 1988/5
Y1 - 1988/5
N2 - The action of vasoactive intestinal peptide (VIP) on Ca2+ -dependent K+ currents, in dissociated mouse lacrimal cells, was investigated using patch clamp techniques. In whole cell recordings, VIP (10-100 pM) increased the magnitude of the Ca2+-dependent K+ current. In single channel recordings, VIP increased the fraction of time the large chary bdotoxin-sensitive Ca2+-activated K+ channel spent in the open state. The activity of this channel was also increased by adding forskolin or 8-bromo cAMP to the bath. Additionally, application of either cAMP or catalytic subunit of cAMP-dependent protein kinase directly to the cytoplasmic surface of excised inside out patches reversibly lengthened the time Ca2+-activated K+ channels spent in the open state. These data suggest that VIP stimulates Ca2+-activated K+ channels by a cAMR-dependent pathway in mouse lacrimal acinar cells.
AB - The action of vasoactive intestinal peptide (VIP) on Ca2+ -dependent K+ currents, in dissociated mouse lacrimal cells, was investigated using patch clamp techniques. In whole cell recordings, VIP (10-100 pM) increased the magnitude of the Ca2+-dependent K+ current. In single channel recordings, VIP increased the fraction of time the large chary bdotoxin-sensitive Ca2+-activated K+ channel spent in the open state. The activity of this channel was also increased by adding forskolin or 8-bromo cAMP to the bath. Additionally, application of either cAMP or catalytic subunit of cAMP-dependent protein kinase directly to the cytoplasmic surface of excised inside out patches reversibly lengthened the time Ca2+-activated K+ channels spent in the open state. These data suggest that VIP stimulates Ca2+-activated K+ channels by a cAMR-dependent pathway in mouse lacrimal acinar cells.
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U2 - 10.1016/0896-6273(88)90143-2
DO - 10.1016/0896-6273(88)90143-2
M3 - Article
C2 - 2856094
AN - SCOPUS:0024002713
SN - 0896-6273
VL - 1
SP - 227
EP - 235
JO - Neuron
JF - Neuron
IS - 3
ER -