Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus

Uday Bandyopadhyay; Thorsten Kaiser; Marco T. Rudolf; Carsten Schultz; Andreas H. Guse; Georg W. Mayr

doi:10.1006/bbrc.1997.7629

Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus

Uday Bandyopadhyay
, Thorsten Kaiser
, Marco T. Rudolf
, Carsten Schultz
, Andreas H. Guse
, Georg W. Mayr

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P₅) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P₅. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P₅ 5-phosphatase activity.

Original language	English (US)
Pages (from-to)	146-149
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	240
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.7629
State	Published - Nov 7 1997
Externally published	Yes

Funding

We are grateful to the Deutsche Forschungsgemeinschaft for a grant (grant GU 360/1-2 to A.H.G and G.W.M). U.B. was supported by the Alexander-von-Humboldt-Foundation (Bonn, Germany).

Funders	Funder number
Alexander-von-Humboldt-Foundation
Deutsche Forschungsgemeinschaft	GU 360/1-2

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus",

abstract = "Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P5. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P5 5-phosphatase activity.",

author = "Uday Bandyopadhyay and Thorsten Kaiser and Rudolf, \{Marco T.\} and Carsten Schultz and Guse, \{Andreas H.\} and Mayr, \{Georg W.\}",

note = "Funding Information: We are grateful to the Deutsche Forschungsgemeinschaft for a grant (grant GU 360/1-2 to A.H.G and G.W.M). U.B. was supported by the Alexander-von-Humboldt-Foundation (Bonn, Germany).",

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doi = "10.1006/bbrc.1997.7629",

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T1 - Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus

AU - Bandyopadhyay, Uday

AU - Kaiser, Thorsten

AU - Rudolf, Marco T.

AU - Schultz, Carsten

AU - Guse, Andreas H.

AU - Mayr, Georg W.

N1 - Funding Information: We are grateful to the Deutsche Forschungsgemeinschaft for a grant (grant GU 360/1-2 to A.H.G and G.W.M). U.B. was supported by the Alexander-von-Humboldt-Foundation (Bonn, Germany).

PY - 1997/11/7

Y1 - 1997/11/7

N2 - Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P5. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P5 5-phosphatase activity.

AB - Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P5. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P5 5-phosphatase activity.

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Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus

Abstract

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ASJC Scopus subject areas

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