Viral restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens

Richard T. Maziarz; Steven J. Burakoff; Carol S. Reiss

doi:10.1016/0008-8749(86)90235-2

Viral restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens

Richard T. Maziarz
, Steven J. Burakoff
, Carol S. Reiss

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (α1 and α2) of H-2K^b and the carboxy-terminal domains (α3, transmembrane, and intracellular) of HLA-A2. Previously we demonstrated that these antigens were serologically intact and were recognized by allospecific cytolytic T lymphocytes. However, the cell lines expressing the hybrid antigen were less well lysed than the native H-2K^b expressing cell lines. In this study, we extend these observations and demonstrate that virally restricted cytolytic T lymphocytes specific for vesicular stomatitis virus and for Sendai virus can recognize cell lines expressing the hybrid antigen, whether expressed on murine (L cell) or human (RD cell) lines. Furthermore, the data show a profound influence by the carboxy-terminal domains upon the polymorphic T-cell restricting epitopes.

Original language	English (US)
Pages (from-to)	279-286
Number of pages	8
Journal	Cellular Immunology
Volume	99
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(86)90235-2
State	Published - Apr 15 1986
Externally published	Yes

Funding

’ This work was supported by the Dyson Foundation, National Institutes of Health Grants AI 18083 and AI 17258, and the American Cancer Society Grant Junior Faculty Research Award (JFRA 107) to C.S.R. 2 To whom correspondence should be addressed: Division of Pediatric Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Mass. 02 115. 3 Abbreviations used: CTL, cytolytic T lymphocytes; MAb, monoclonal antibody; MHC, major histocompatibility complex; neo, neomycin resistance; VSV, vesicular stomatis virus.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	AI 18083
American Cancer Society-Stanford Cancer Institute	JFRA 107
National Institute of Allergy and Infectious Diseases	R01AI017258
Dana Farber Cancer Institute
Dyson Foundation

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(86)90235-2

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@article{000eea4a0b604e03aa48ea8f25d8dfca,

title = "Viral restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens",

abstract = "Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (α1 and α2) of H-2Kb and the carboxy-terminal domains (α3, transmembrane, and intracellular) of HLA-A2. Previously we demonstrated that these antigens were serologically intact and were recognized by allospecific cytolytic T lymphocytes. However, the cell lines expressing the hybrid antigen were less well lysed than the native H-2Kb expressing cell lines. In this study, we extend these observations and demonstrate that virally restricted cytolytic T lymphocytes specific for vesicular stomatitis virus and for Sendai virus can recognize cell lines expressing the hybrid antigen, whether expressed on murine (L cell) or human (RD cell) lines. Furthermore, the data show a profound influence by the carboxy-terminal domains upon the polymorphic T-cell restricting epitopes.",

author = "Maziarz, \{Richard T.\} and Burakoff, \{Steven J.\} and Reiss, \{Carol S.\}",

note = "Funding Information: {\textquoteright} This work was supported by the Dyson Foundation, National Institutes of Health Grants AI 18083 and AI 17258, and the American Cancer Society Grant Junior Faculty Research Award (JFRA 107) to C.S.R. 2 To whom correspondence should be addressed: Division of Pediatric Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Mass. 02 115. 3 Abbreviations used: CTL, cytolytic T lymphocytes; MAb, monoclonal antibody; MHC, major histocompatibility complex; neo, neomycin resistance; VSV, vesicular stomatis virus.",

year = "1986",

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day = "15",

doi = "10.1016/0008-8749(86)90235-2",

language = "English (US)",

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pages = "279--286",

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T1 - Viral restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens

AU - Maziarz, Richard T.

AU - Burakoff, Steven J.

AU - Reiss, Carol S.

N1 - Funding Information: ’ This work was supported by the Dyson Foundation, National Institutes of Health Grants AI 18083 and AI 17258, and the American Cancer Society Grant Junior Faculty Research Award (JFRA 107) to C.S.R. 2 To whom correspondence should be addressed: Division of Pediatric Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Mass. 02 115. 3 Abbreviations used: CTL, cytolytic T lymphocytes; MAb, monoclonal antibody; MHC, major histocompatibility complex; neo, neomycin resistance; VSV, vesicular stomatis virus.

PY - 1986/4/15

Y1 - 1986/4/15

N2 - Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (α1 and α2) of H-2Kb and the carboxy-terminal domains (α3, transmembrane, and intracellular) of HLA-A2. Previously we demonstrated that these antigens were serologically intact and were recognized by allospecific cytolytic T lymphocytes. However, the cell lines expressing the hybrid antigen were less well lysed than the native H-2Kb expressing cell lines. In this study, we extend these observations and demonstrate that virally restricted cytolytic T lymphocytes specific for vesicular stomatitis virus and for Sendai virus can recognize cell lines expressing the hybrid antigen, whether expressed on murine (L cell) or human (RD cell) lines. Furthermore, the data show a profound influence by the carboxy-terminal domains upon the polymorphic T-cell restricting epitopes.

AB - Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (α1 and α2) of H-2Kb and the carboxy-terminal domains (α3, transmembrane, and intracellular) of HLA-A2. Previously we demonstrated that these antigens were serologically intact and were recognized by allospecific cytolytic T lymphocytes. However, the cell lines expressing the hybrid antigen were less well lysed than the native H-2Kb expressing cell lines. In this study, we extend these observations and demonstrate that virally restricted cytolytic T lymphocytes specific for vesicular stomatitis virus and for Sendai virus can recognize cell lines expressing the hybrid antigen, whether expressed on murine (L cell) or human (RD cell) lines. Furthermore, the data show a profound influence by the carboxy-terminal domains upon the polymorphic T-cell restricting epitopes.

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Viral restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens

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