Visualization of virus-infected brain regions using a GFP-illuminating flashlight enables accurate and rapid dissection for biochemical analysis

Xuan Li; Marina E. Wolf

doi:10.1016/j.jneumeth.2011.07.016

Visualization of virus-infected brain regions using a GFP-illuminating flashlight enables accurate and rapid dissection for biochemical analysis

Xuan Li, Marina E. Wolf

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Engineered viral vectors tagged with a fluorescent protein such as enhanced green fluorescent protein (EGFP) have been widely used to study neuronal function after intracranial injection into specific brain regions. A rapid dissection of the virus-expressing region is required for certain biochemical analyses. To improve the accuracy of the rapid dissection, we developed a method that employs a Bluestar flashlight in combination with barrier filter glasses to visualize the expression of EGFP lentivirus that has been microinjected into the nucleus accumbens. Processing of dissected tissue for EGFP immunoblotting further validated the approach.

Original language	English (US)
Pages (from-to)	177-179
Number of pages	3
Journal	Journal of Neuroscience Methods
Volume	201
Issue number	1
DOIs	https://doi.org/10.1016/j.jneumeth.2011.07.016
State	Published - Sep 30 2011
Externally published	Yes

Keywords

Bluestar flashlight
EGFP
Lentivirus
Tissue dissection
Viral vector

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.jneumeth.2011.07.016

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abstract = "Engineered viral vectors tagged with a fluorescent protein such as enhanced green fluorescent protein (EGFP) have been widely used to study neuronal function after intracranial injection into specific brain regions. A rapid dissection of the virus-expressing region is required for certain biochemical analyses. To improve the accuracy of the rapid dissection, we developed a method that employs a Bluestar flashlight in combination with barrier filter glasses to visualize the expression of EGFP lentivirus that has been microinjected into the nucleus accumbens. Processing of dissected tissue for EGFP immunoblotting further validated the approach.",

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AU - Wolf, Marina E.

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AB - Engineered viral vectors tagged with a fluorescent protein such as enhanced green fluorescent protein (EGFP) have been widely used to study neuronal function after intracranial injection into specific brain regions. A rapid dissection of the virus-expressing region is required for certain biochemical analyses. To improve the accuracy of the rapid dissection, we developed a method that employs a Bluestar flashlight in combination with barrier filter glasses to visualize the expression of EGFP lentivirus that has been microinjected into the nucleus accumbens. Processing of dissected tissue for EGFP immunoblotting further validated the approach.

KW - Bluestar flashlight

KW - EGFP

KW - Lentivirus

KW - Tissue dissection

KW - Viral vector

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Visualization of virus-infected brain regions using a GFP-illuminating flashlight enables accurate and rapid dissection for biochemical analysis

Abstract

Keywords

ASJC Scopus subject areas

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