What is normal? A characterization of the values and variability in reproductive endpoints of the fathead minnow, Pimephales promelas

Karen H. Watanabe; Kathleen M. Jensen; Edward F. Orlando; Gerald T. Ankley

doi:10.1016/j.cbpc.2007.04.015

What is normal? A characterization of the values and variability in reproductive endpoints of the fathead minnow, Pimephales promelas

Karen H. Watanabe
, Kathleen M. Jensen
, Edward F. Orlando
, Gerald T. Ankley

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Jensen et al. [Jensen, K.M., Korte, J.J., Kahl, M.D., Pasha, M.S., Ankley, G.T., 2001. Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas). Comp. Biochem. Physiol. C 128, 127-141.] investigated aspects of the normal reproductive biology of the fathead minnow (FHM, P. promelas), and subsequent studies have generated a large amount of additional reproductive data for endpoints such as plasma steroid hormone and vitellogenin concentrations, spawn interval, and secondary sex characteristics (i.e., nuptial tubercle score and fat pad weight). These data were analyzed and fitted with statistical distributions to improve understanding of the variability in normal, unexposed, adult male (n = 154) and female (n = 186) FHM. Summary statistics for most endpoints were consistent with results from other more limited studies of FHMs. Male fat pad weight, and in both sexes gonad and liver weights were found to be proportional to body weight. Multiple statistical distributions were found to characterize each endpoint with the exception of spawn interval. Based on one of the largest datasets ever compiled for controlled studies, results presented herein provide a robust point of reference for the quantitative assessment of reproductive processes in the fathead minnow.

Original language	English (US)
Pages (from-to)	348-356
Number of pages	9
Journal	Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume	146
Issue number	3
DOIs	https://doi.org/10.1016/j.cbpc.2007.04.015
State	Published - Sep 2007

Funding

The authors gratefully acknowledge Ms. Zhenhong Li who assisted with calculating spawn intervals. This research was supported in part by a Cooperative Agreement to the University of Florida, FL (N. Denslow), Oregon Health & Science University, OR (KW), Purdue University, IN (M. Sepulveda) and Florida Atlantic University, FL (EO) from the US Environmental Protection Agency (EPA) Science to Achieve Results program (STAR; Assistance Agreement # RD-83184801-0). This research was also supported by resources from the National Center for Computational Toxicology of the EPA Office of Research and Development, awarded to the Ecological Exposure Research and Ecosystem Research Divisions (National Exposure Research Laboratory) in Cincinnati, OH and Athens, GA, respectively, and the Mid-Continent Ecology Division (National Health and Environmental Effects Research Laboratory) in Duluth, MN.

Funders	Funder number
Ecological Exposure Research and Ecosystem Research Divisions
National Exposure Research Laboratory
U.S. Environmental Protection Agency
Research Foundation for Health and Environmental Effects
Indiana University-Purdue University
Oregon State University/Oregon Health and Science University
Florida Atlantic University
Star Scientific Foundation	RD-83184801-0
U.S. Environmental Protection Agency

Keywords

Biological variability
Fathead minnow
Reproduction
Steroid hormones

ASJC Scopus subject areas

Biochemistry
Physiology
Toxicology
Cell Biology
Health, Toxicology and Mutagenesis

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10.1016/j.cbpc.2007.04.015

Cite this

What is normal? A characterization of the values and variability in reproductive endpoints of the fathead minnow, Pimephales promelas. / Watanabe, Karen H.; Jensen, Kathleen M.; Orlando, Edward F. et al.
In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 146, No. 3, 09.2007, p. 348-356.

Research output: Contribution to journal › Article › peer-review

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abstract = "Jensen et al. [Jensen, K.M., Korte, J.J., Kahl, M.D., Pasha, M.S., Ankley, G.T., 2001. Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas). Comp. Biochem. Physiol. C 128, 127-141.] investigated aspects of the normal reproductive biology of the fathead minnow (FHM, P. promelas), and subsequent studies have generated a large amount of additional reproductive data for endpoints such as plasma steroid hormone and vitellogenin concentrations, spawn interval, and secondary sex characteristics (i.e., nuptial tubercle score and fat pad weight). These data were analyzed and fitted with statistical distributions to improve understanding of the variability in normal, unexposed, adult male (n = 154) and female (n = 186) FHM. Summary statistics for most endpoints were consistent with results from other more limited studies of FHMs. Male fat pad weight, and in both sexes gonad and liver weights were found to be proportional to body weight. Multiple statistical distributions were found to characterize each endpoint with the exception of spawn interval. Based on one of the largest datasets ever compiled for controlled studies, results presented herein provide a robust point of reference for the quantitative assessment of reproductive processes in the fathead minnow.",

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note = "Funding Information: The authors gratefully acknowledge Ms. Zhenhong Li who assisted with calculating spawn intervals. This research was supported in part by a Cooperative Agreement to the University of Florida, FL (N. Denslow), Oregon Health \& Science University, OR (KW), Purdue University, IN (M. Sepulveda) and Florida Atlantic University, FL (EO) from the US Environmental Protection Agency (EPA) Science to Achieve Results program (STAR; Assistance Agreement \# RD-83184801-0). This research was also supported by resources from the National Center for Computational Toxicology of the EPA Office of Research and Development, awarded to the Ecological Exposure Research and Ecosystem Research Divisions (National Exposure Research Laboratory) in Cincinnati, OH and Athens, GA, respectively, and the Mid-Continent Ecology Division (National Health and Environmental Effects Research Laboratory) in Duluth, MN. ",

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AU - Ankley, Gerald T.

N1 - Funding Information: The authors gratefully acknowledge Ms. Zhenhong Li who assisted with calculating spawn intervals. This research was supported in part by a Cooperative Agreement to the University of Florida, FL (N. Denslow), Oregon Health & Science University, OR (KW), Purdue University, IN (M. Sepulveda) and Florida Atlantic University, FL (EO) from the US Environmental Protection Agency (EPA) Science to Achieve Results program (STAR; Assistance Agreement # RD-83184801-0). This research was also supported by resources from the National Center for Computational Toxicology of the EPA Office of Research and Development, awarded to the Ecological Exposure Research and Ecosystem Research Divisions (National Exposure Research Laboratory) in Cincinnati, OH and Athens, GA, respectively, and the Mid-Continent Ecology Division (National Health and Environmental Effects Research Laboratory) in Duluth, MN.

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KW - Biological variability

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KW - Reproduction

KW - Steroid hormones

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What is normal? A characterization of the values and variability in reproductive endpoints of the fathead minnow, Pimephales promelas

Abstract

Funding

Keywords

ASJC Scopus subject areas

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