White matter lesions defined by diffusion tensor imaging in older adults

Stephen A. Back, Christopher D. Kroenke, Larry S. Sherman, Gus Lawrence, Xi Gong, Erin N. Taber, Joshua A. Sonnen, Eric B. Larson, Thomas J. Montine

Research output: Contribution to journalArticlepeer-review

94 Scopus citations


Objective: The cellular and molecular mechanisms underlying magnetic resonance imaging-defined white matter (WM) changes associated with age-related cognitive decline remain poorly defined. We tested the hypothesis that WM lesions in older adults, defined by diffusion tensor imaging (DTI), arise in the setting of vascular brain injury (VBI) and are characterized by increased free radical injury and aberrant oligodendrocyte lineage (OL) cell response to injury. Methods: We undertook a multimodal analysis of prefrontal cortex (PFC) WM from 25 autopsies derived from a population-based cohort where VBI and Alzheimer disease (AD) frequently coincide. Ex vivo high field strength DTI measurements of fractional anisotropy (FA), apparent diffusion coefficient, and axial and radial (D) diffusivity were measured at high magnetic field strength (11.7T) and analyzed relative to quantitative in vivo biomarkers of free radical injury, an OL-specific marker Olig2, and histologic evaluation of hyaluronan (HA), an inhibitor of OL maturation. Results: Coincident AD and VBI showed significant association with lower FA and a robust relationship between decreasing FA and increasing D. Free radical injury to docosahexaenoate and adrenate in PFC WM was significantly elevated in cases with VBI independent of AD, and was inversely correlated with FA. Similarly, increased density of Olig2-immunoreactive cells in PFC WM was significantly associated with VBI independent of AD and colocalized with regions enriched in HA. Interpretation: DTI-defined PFC WM lesions in older individuals are characterized by free radical injury to myelin and neuroaxonal elements that coincides with pronounced expansion of the pool of OL cells in HA-rich regions.

Original languageEnglish (US)
Pages (from-to)465-476
Number of pages12
JournalAnnals of Neurology
Issue number3
StatePublished - Sep 2011

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology


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