TY - JOUR
T1 - Widespread presence of histologically occult cytomegalovirus
AU - Myerson, David
AU - Hackman, Robert C.
AU - Nelson, Jay A.
AU - Ward, David C.
AU - McDougall, James K.
N1 - Funding Information:
To date, the isolation of virus in tissue culture, with identification of the attendant cytologic changes, thology Divisions, Fred Hutchinson Cancer Research Center, Seattle, Washington, and the :~Departments of Human Genetics and Molecular Biophysics-Biochemistry, Yale University .School of Medicine, New Haven, Connecticut. Supported by Human Cancer-directed Fellowship DRG-003 of the Damon Runyon-Walter Win-chell Cancer Fund (D. M.), a Junior-Faculty Clinical Fellowship from ihe American Cancer Society (R. C. H.), Public Health Service Fellowship CA 06986 r A. N.), and grants from the March of Dimes (I-769, J. K. McD.) and the National Institutes of Health (GM 20124, D. C. W.; CA 29350,J. K. McD.; and CA 18029). Accepted for publication May 19, 1983.
PY - 1984/5
Y1 - 1984/5
N2 - Disseminated cytomegalovirus (CMV) has been investigated by in situ hybridization in formalin-fixed paraffin-embedded tissue sections with biotinylated DNA probes. Two cases of disseminated CMV infection were studied at autopsy by this highly specific technique. The presence of CMV in cytomegalic cells is readily shown. In addition, CMV has been detected and localized in many normal-appearing cells. This occult infection occurs in cardiac myocytes, hepatocytes, spleen and lymph node reticular cells, endometrial stromal and glandular cells, and breast stromal cells, as well as in cells in the renal glomerulus, tubule, and interstitium, adrenal cortex and medulla, fallopian tube submucosa, myometrium, and anterior pituitary. Cytomegalovirus infection of endothelial cells has been further documented by immunohistochemical methods utilizing antibody to Factor VIII. These findings suggest that CMV disseminates hematogenously throughout the body, initiating necrotizing foci of infection. The appearance of many diffuse foci suggests that local viral spread occurs via endothelial cell infection. Surprisingly, lymphocyte involvement was not observed.
AB - Disseminated cytomegalovirus (CMV) has been investigated by in situ hybridization in formalin-fixed paraffin-embedded tissue sections with biotinylated DNA probes. Two cases of disseminated CMV infection were studied at autopsy by this highly specific technique. The presence of CMV in cytomegalic cells is readily shown. In addition, CMV has been detected and localized in many normal-appearing cells. This occult infection occurs in cardiac myocytes, hepatocytes, spleen and lymph node reticular cells, endometrial stromal and glandular cells, and breast stromal cells, as well as in cells in the renal glomerulus, tubule, and interstitium, adrenal cortex and medulla, fallopian tube submucosa, myometrium, and anterior pituitary. Cytomegalovirus infection of endothelial cells has been further documented by immunohistochemical methods utilizing antibody to Factor VIII. These findings suggest that CMV disseminates hematogenously throughout the body, initiating necrotizing foci of infection. The appearance of many diffuse foci suggests that local viral spread occurs via endothelial cell infection. Surprisingly, lymphocyte involvement was not observed.
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U2 - 10.1016/S0046-8177(84)80076-3
DO - 10.1016/S0046-8177(84)80076-3
M3 - Article
C2 - 6327494
AN - SCOPUS:0021671099
SN - 0046-8177
VL - 15
SP - 430
EP - 439
JO - Human Pathology
JF - Human Pathology
IS - 5
ER -