Abstract
Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.
Original language | English (US) |
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Pages (from-to) | 2537-2546 |
Number of pages | 10 |
Journal | Nucleic acids research |
Volume | 8 |
Issue number | 11 |
DOIs | |
State | Published - Jun 11 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics